Publication: B12-based photoreceptors: from structure and function to applications in optogenetics and synthetic biology
Authors
Pérez-Castaño, Ricardo ; Elías Arnanz, Montserrat ; Padmanabhan Iyer, Subramanian
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DOI
10.1016/j.sbi.2019.01.020
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info:eu-repo/semantics/article
Description
Abstract
Vitamin B12-based photoreceptor proteins sense ultraviolet (UV), blue or green light using 5´-deoxyadenosylcobalamin (AdoCbl). The prototype of this widespread bacterial photoreceptor family, CarH, controls light-dependent gene expression in photoprotective cellular responses. It represses transcription in the dark by binding to operator DNA as an AdoCbl-bound tetramer, whose disruption by light relieves operator binding to allow transcription. Structures of the "dark" (free and DNA-bound) and "light" CarH states and studies on the unusual AdoCbl photochemistry have provided fundamental insights into these photoreceptors. We highlight these, the plasticity within a conserved mode of action among CarH homologs, their distribution, and their promising applications in optogenetics and synthetic biology.
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Citation
Current Opinion in Structural Biology 2019, 57:47-55
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