Publication: Tyrosine hydroxylase in mouse pancreatic islet cells,
in situ and after syngeneic transplantation to kidney
Authors
Persson-Sjögren, S. ; Forsgren, Sture ; Täljedal, I.B.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
Tyrosine hydroxylase (TH) is co-expressed
with islet hormones in the fetal mouse pancreas. In the
adult animal, the enzyme has been considered as a
marker of ageing ß-cells. By immunohistochemical
staining, we analyzed the expression of TH-like
immunoreactivity (TH-LI), insulin-LI (INS-LI) and
somatostatin-LI (SOM-LI) in adult mouse islets, in situ
and after isolation and transplantation to kidney. In
pancreas in situ, most TH-LI cells expressed INS-LI
while less than 5% expressed SOM-LI. The total number
of TH-LI cells/mm2 was significantly increased directly
after isolation and in 0-day, 12-week and 52-week old
grafts, but not in 3-day grafts. The proportion of TH-LI
cells expressing SOM-LI increased after transplantation,
amounting to about one-third by 52 weeks. As expressed
per unit islet area, the frequencies of both TH/INS and
TH/SOM cells increased significantly in the transplants.
The results demonstrate that TH occurs in both ß-cells
and D-cells of adult islets. In both cell types the enzyme
appears to be responsive to the microenvironmental
changes inherent in transplantation. This cellular
phenotype plasticity might contribute to the altered
insulin secretory dynamics in islet grafts.
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