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Quantitative mRNA expression analysis in kidney glomeruli using microdissection techniques

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dc.contributor.authorDe Spiegelaere, Ward
dc.contributor.authorCornillie, Pieter
dc.contributor.authorVan Poucke, Mario
dc.contributor.authorPeelman, Luc
dc.contributor.authorBurvenich, Christian
dc.contributor.authorVan den Broeck, Wim
dc.date.accessioned2016-02-09T14:38:08Z
dc.date.available2016-02-09T14:38:08Z
dc.date.issued2011
dc.description.abstractThe introduction of new tools for molecular analysis, such as RT-qPCR and microarrays, has provided researchers with powerful applications to study renal disease and development. However, the high cellular heterogeneity of the renal tissue complicates the molecular analysis of specific cells and cell groups such as glomerular or tubular cells. In the past, glomerular sieving and manual dissection were used for the isolation of glomeruli. However, these techniques cannot be used for the isolation of specific glomeruli or for the coisolation of additional tissue fractions. In recent decades, new microdissection techniques such as laser-assisted microdissection have been developed. These applications allow the isolation of small cell groups from heterogeneous tissue for molecular analysis, including microarray and RT-qPCR. Although very promising, some drawbacks are associated with these techniques. The isolated sample material is generally small and requires sensitive assays. In addition, the long sample processing time may result in a considerable loss of RNA integrity. Careful optimization and rigorous quality analysis should overcome these drawbacks. In the present paper, the recent literature on the application of microdissection techniques in kidney research is reviewed, together with a discussion of the critical issues that are essential for the application of quantitative mRNA expression analysis with RT-qPCR on microdissected samples.es
dc.formatapplication/pdfes
dc.format.extent9es
dc.identifier.issn1699-5848
dc.identifier.issn0213-3911
dc.identifier.urihttp://hdl.handle.net/10201/47821
dc.languageenges
dc.publisherMurcia: F. Hernándezes
dc.relation.ispartofHistology and histopathology, Vol. 26, nº 2 (2011)es
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.subjectKidneyes
dc.subjectGene expression analysises
dc.subject.other612 - Fisiologíaes
dc.titleQuantitative mRNA expression analysis in kidney glomeruli using microdissection techniqueses
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
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Histology and histopathology Vol.26, nº2 (2011)