Publication: Immunohistochemical localization of truncated midkine in developing human bile ducts
Authors
Kato, Massuo J. ; Shinozawa, T. ; Kato, S. ; Terada, T.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
Midkine (MK) is a heparin-binding growth
factor whose gene has been identified in embryonal
carcinoma cells in early stages of retinoic acid-induced
differentiation. In the present study, we investigated the
developmental localization of truncated MK protein in
human bile ducts. Thirty specimens of the livers from 25
fetuses (from 9 to 40 gestational weeks) and from five
neonates less than 4 weeks old were examined.
Immunohistochemical analysis was performed using a
mouse IgG2b monoclonal antibody against recombinanttruncated
MK. Truncated MK was expressed moderately
in the fetal liver from 9 to 15 gestational weeks. The
immunoreactivities were found in the primitive
hepatocytes, ductal plates, migrating biliary cells and
immature bile ducts. The reaction products were
localized in the cytoplasm heterogeneously. The
intensity of immunostaining was weak from 15
gestational weeks to 26 gestational weeks. After 27
gestational weeks, truncated MK was not detected in the
fetal livers. It was suggested that primitive hepatocytes,
ductal plates and immature bile ducts produced truncated
MK transiently during human bile ducts development
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