Publication: Differential effects of intestinal ischemia
and reperfusion in rat enteric neurons
and glial cells expressing P2X2 receptors
Authors
Marosti, Aline Rosa ; Da Silva, Marcos Vinícius ; Palombit, Kelly ; Mendes, Cristina Eusébio ; Tavares de Lima, Wothan ; Castelucci, Patricia
item.page.secondaryauthor
item.page.director
Publisher
F. Hernández y Juan F. Madrid. Universidad de Murcia: Departamento de Biología Celular e Histología
publication.page.editor
publication.page.department
DOI
https://doi.org/10.14670/HH-30.489
item.page.type
info:eu-repo/semantics/article
Description
Abstract
Background. Intestinal ischemia followed by
reperfusion (I/R) may occur following intestinal
obstruction. In rats, I/R in the small intestine leads to
structural changes accompanied by neuronal death.
Aim. The objective was to analyze the impact of I/R
injury on different neuronal populations in the myenteric
plexus of the rat ileum after different periods of
reperfusion.
Methods. The superior mesentery artery was
occluded for 45 minutes, and animals were euthanized
after 24 hours and 1 week of reperfusion. Immunohistochemical analyses were performed with antibodies
against the P2X2 receptor in combination with
antibodies against nitric oxide synthase (NOS), choline
acetyltransferase (ChAT), calbindin, calretinin, the panneuronal marker anti-HuC/D, or S100β (glial marker).
Results. Dual immunolabeling demonstrated that
approximately 100% of NOS-, ChAT-, calbindin-, and
calretinin-immunoreactive neurons in all groups
expressed the P2X2 receptor. Following I/R, the
neuronal density decreased in the P2X2 receptor-, ChAT-
, calretinin-, and HuC/D-immunoreactive neurons at 24
hours and 1 week following injury compared to the
densities in the control and sham groups. The calbindinimmunoreactive neuron density was not reduced in any
of the groups. The density of enteric glial cells increased
by 40% in the I/R group compared to the density in the
sham groups. We also observed increases of 12%, 16%,
and 23% in the neuronal cell body profile areas of the
NOS-, ChAT-, and calbindin-immunoreactive neurons,
respectively, at 1 week following I/R. However, the
average size of the calretinin-immunoreactive neurons
was reduced by 12% in the I/R group at 24 hours.
Conclusions This work demonstrates that I/R is
associated with a significant loss of different classes of
neurons in the myenteric plexus accompanied by
morphological changes and an increased density of
enteric glial cells; all of these effects may underlie
conditions related to intestinal motility disorder.
publication.page.subject
Citation
Histology and Histopathology, Vol. 30, n.º 4 (2015)
item.page.embargo
Ir a Estadísticas
Este ítem está sujeto a una licencia Creative Commons. http://creativecommons.org/licenses/by-nc-nd/4.0/