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The development of bone changes induced in rats by recombinant human granulocyte colonystimulating factor is suppressed by bisphosphonate

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dc.contributor.authorSuzuki, M.es
dc.contributor.authorAdachi, K.es
dc.contributor.authorSugimoto, T.
dc.contributor.authorNakayama, Hiroyuki
dc.contributor.authorDoi, K.
dc.date.accessioned2011-02-22T11:09:20Z
dc.date.available2011-02-22T11:09:20Z
dc.date.issued1999
dc.description.abstractWe have previously demonstrated that high doses of recombinant human granulocyte colonystimulating factor (rhG-CSF) induce bone changes characterized by osteoclastic bone resorption and osteogenesis due to intramembranous ossification in rats. In this communication we examined the effects of a pretreatment with 3-amino-l-hydroxypropylidene-1,lbisphosphonate (AHPrBP), which is a powerful inhibitor of osteoclastic bone resorption, on bone changes induced by rhG-CSF in order to investigate the relation between osteoclastic bone resorption and osteogenesis. AHPrBP (5 mg/kg/day) was subcutaneously given to 6-week-old rats for 2 days. From the following day of the final injection of AHPrBP, rats received a subcutaneous injection of rhG-CSF (1,000 pg/kg/day) for 14 days, and the femur and tibia were evaluated histopathologically. By the analysis of peripheral blood leukocyte counts, spleen weights and bone marrow findings, the pretreatment with AHPrBP had no effect on the activation of hematopoiesis related to the major pharmacological activity of rhG-CSF. In the rats treated with rhG-CSF alone, accelerated osteoclastic bone resorption and osteogenesis due to intramernbranous ossification were observed in the trabeculae of metaphyseal spongiosa. The accelerated osteoclastic bone resorption induced by rhG-CSF was suppressed by the pharmacological activity of AHPrBP. Furthermore, the osteogenesis induced by rhG-CSF was also suppressed by AHPrBP. These results suggest that the osteogenesis induced by rhG-CSF is a sequential reaction of accelerated osteoclastic bone resorption, and moreover that the main action of rhG-CSF on bone is an acceleration of osteoclastic bone resorption.es
dc.formatapplication/pdfes
dc.format.extent8es
dc.identifier.issn0213-3911es
dc.identifier.urihttp://hdl.handle.net/10201/19206
dc.languageenges
dc.publisherMurcia : F. Hernándezes
dc.relation.ispartofHistology and histopathologyen_EN
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.subjectOsteogenesisen_EN
dc.subjectBisphosphonatesen_EN
dc.subject.otherCDU::6 - Ciencias aplicadas::63 - Agricultura. Silvicultura. Zootecnia. Caza. Pesca::636 - Veterinaria. Explotación y cría de animales. Cría del ganado y de animales domésticoses
dc.titleThe development of bone changes induced in rats by recombinant human granulocyte colonystimulating factor is suppressed by bisphosphonateen_EN
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
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Histology and histopathology Vol.14, nº 3 (1999)