Publication: Effects of the isoflavone daidzein in Senegalese sole, Solea senegalensis: Modulation of the oestrogen receptor-β, apoptosis and enzymatic signalling pathways
Authors
Sarasquete, Carmen ; Úbeda Manzanaro, María ; Ortiz Delgado, Juan B.
item.page.secondaryauthor
item.page.director
Publisher
Universidad de Murcia. Departamento de Biología Celular e Histología
publication.page.editor
publication.page.department
DOI
DOI: 10.14670/HH-18-090
item.page.type
info:eu-repo/semantics/article
Description
Abstract
Phytochemicals are widely present in the
aquatic environment and they are derived from many
anthropogenic activities. The isoflavone daidzein is a
natural compound that is found in the soya products used
as habitual constituents of aquafeeds. Nevertheless, this
isoflavone possesses oestrogenic and apoptotic
properties. The present study determined the effects of
daidzein (at 20 mg/L) during the first month and a half
of life (from 7 to 44 days post-hatching -dph-) of the
flatfish Senegalese sole, Solea senegalensis, focusing at
the metamorphosis. We have analysed different gene
expression levels and immunohistochemical protein
patterns implicated in some oestrogenic, apoptotic and
enzymatic pathways. In general, the oestrogen receptor
(ERβ) and stimulating apoptosis death receptor factor
(Fas) transcript levels showed similar baseline patterns
and transcriptional responses induced by daidzein. Both
ERβ and Fas were up-regulated by this isoflavone at the
pre-metamorphosis and metamorphosis, and they were
down-regulated in post-metamorphosed stages. The
expression pattern of the apoptotic effector caspase
(Casp6) was exclusively up-regulated at the premetamorphic phase. The Birc5 transcripts (i.e. antiapoptosis, Survivin) were down-regulated by daidzein
during certain metamorphic and post-metamorphosed
stages. Besides, daidzein showed an up-regulating effect
on both enzymatic complexes, the haemoprotein CYP1A
and the acetylcholinesterase (AChE), except for a
temporary AChE down-regulation in some postmetamorphosed stages. Immunostaining analysis only
showed increased CYP1A signals in the liver of daidzein
exposed fish. Overall, a majority of the transcriptional
oestrogenic and apoptotic imbalances could be gradually
and/or temporarily stabilised. Most controls and exposed
larvae (70-80%) developed and grew following normal
ontogenetic developmental patterns.
publication.page.subject
AChE , Apoptosis , CYP1A , ERβ , Daidzein , Senegalese sole , Survivin , Immunohistochemistry , Metamorphosis
Citation
Histology and Histopathology, Vol.34, nº8, (2019)
item.page.embargo
Ir a Estadísticas
Este ítem está sujeto a una licencia Creative Commons. http://creativecommons.org/licenses/by-nc-nd/4.0/