Publication: Expression of proliferation marker Ki67 correlates to occurrence of metastasis and prognosis, histological subtypes and HPV DNA detection in penile carcinomas
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Date
2007
Authors
Protzel, C. ; Knoedel, J. ; Zimmermann, U. ; Woenckhaus, C. ; Poetsch, M. ; Giebel, J.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
Clinical outcome of penile squamous cell
carcinoma (PSCC) largely depends on the presence of
lymph node metastasis. In search of a valuable marker
predicting the risk for metastasis, the expression of Ki67
was investigated immunohistochemically in primary
tumors and compared to presence of inguinal lymph
node metastasis. As human papilloma virus (HPV) is
thought to affect Ki67 expression, we evaluated whether
occurrence of HPV DNA correlates to Ki67 score or
metastatic potential.
Samples originated from patients subjected to
resection of invasive SCC of penis. Immunohistochemistry
was done on paraffin-embedded sections
using a monoclonal antibody against Ki67. After DNA
isolation from paraffin embedded tissue the presence of
HPV 6/11, HPV 16 and HPV 18 DNA was analyzed by
PCR. Statistical analysis was done using two tail
unpaired t test and Chi-square test.
Four of 28 patients showed a weak Ki67 expression,
without displaying lymph node metastasis. Among 17
patients showing an intermediate Ki67 index, eight
exhibited metastases while in all seven patients with a
strong expression of Ki67 lymph node metastases were
found. The median Ki67 expression in metastastic
lesions was significantly different (50.3%) from tumors
without lymph node metastasis (31.8%) (p=0.024).
Furthermore, a correlation between presence of HPV
DNA and strong Ki67 expression was determined
(p=0.009).
Since our study demonstrated a strong Ki67 labeling
index significantly associated to positive lymph nodes,
we suggest Ki67 expression as a prognostic marker for
lymph node metastasis in penile squamous carcinoma.
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