Publication: Alterations induced by E-cadherin
and ß-catenin antibodies during the
development of Buto arenarum (Anura-Bufonidae)
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Date
2001
Authors
Izaguirre, M. F. ; Adur, J. F. ; Peralta Soler, A. ; Casco, V. H.
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Publisher
F. Hernández y Juan F. Madrid. Universidad de Murcia: Departamento de Biología Celular e Histología
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DOI
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info:eu-repo/semantics/article
Description
Abstract
E(e pi theli al)-ca dh erin is a member of a
ca lcium-dependent famil y of cell surface glycoproteins
in vo lved in ce ll- ce ll adh esion and mo rph oge nesis.
Catenins are a large family of proteins that connect the
cad herins to the cytoskeleton. They are import ant for
cadherin fUll ction and for transducing signals involved in
specification of ce ll fate during embryogenesis. The best
charac teri zed ca tenin s in clude U - , /3- , Yo, and p1 20-
ca tenin. Usin g spec ifi c antibodi es, we studi ed th e
ex pression and distribution of E-cadherin, and u- and /3 -
catenin in developmental stages of Bufo arellarum toad.
The three proteins were fo und co-locali zed in stages 19
to 41 of development. Surprisingly, E-cadherin was the
only of these three proteins fo und earlier than stage 19.
To test whe th e r E-ca dh e rin a nd /3- ca te nin have a
functional role in B/./fo arenarlIIn embryogenesis, stage
17 whole embryos were incubated with anti-E-cadherin
and /3-catenin antibodies. Both anti-E-cadherin and anti-
/3- ca tenin antibodi es indu ced seve re morph ological
alterations. However, while alterations produced by the
anti-/3 -catenin antibody, showed some vari ability from
the most severe (neural tube and notochord duplication)
to a simple delay in deve lopment, the alterations with
anti-E-cadherin were homogeneous. These observa tions
suggest a critica l role for E-cadherin and/3-catenin in the
ea rl y embryo ni c development of the Bufo arenarum
toad. Our resul ts are consistent with the development al
role of these proteins in other species. One of the most
surprising findin gs was th e blockage with the anti-Bcat enin antibodi es on late r embr yo stages, and we
hypothesize that the partial axes duplica tion could be
medi ated by the notochord induction.
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Citation
Histology and histopathology, Vol. 16, nº 4 (2001)
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