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The healing of alkali-injured cornea is stimulated by a novel matrix regenerating agent (RGTA, CACICOL20) - a biopolymer mimicking heparan sulfates reducing proteolytic, oxidative and nitrosative damage

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dc.contributor.authorCejkova, J.
dc.contributor.authorOlmiere, C.
dc.contributor.authorCejka, C.
dc.contributor.authorTrosan, P.
dc.contributor.authorHolan, V.
dc.date.accessioned2019-02-27T09:55:12Z
dc.date.available2019-02-27T09:55:12Z
dc.date.issued2014
dc.description.abstractThe efficacy of a chemically modified dextran - heparan sulfate mimicking regenerating agent (RGTA) on the healing of the rabbit cornea injured with alkali was examined. The eyes were injured with 0.15 N NaOH applied on the cornea or with 1.0 N NaOH using a 8 mm diameter filter paper disk. Then RGTA or placebo was applied on the cornea. In the last group of rabbits, corneas injured with the high alkali concentration were left without any treatment for four weeks; subsequently, the corneas were treated with RGTA or placebo. The central corneal thickness was measured using a pachymeter. The corneas were examined morphologically, immunohistochemically and for real time-PCR. Compared to control (unaffected) corneas, following the application of low alkali concentration the expression of urokinase-type plasminogen activator, metalloproteinase 9, nitric oxide synthase and xanthine oxidase was increased in the injured corneal epithelium of placebo-treated eyes, whereas the expression of antioxidant enzymes was reduced. Nitrotyrosine and malondialdehyde stainings appeared in the corneal epithelium. RGTA application suppressed the antioxidant/prooxidant imbalance and reduced the expression of the above-mentioned immunohistochemical markers. The corneal thickness increased after alkali injury, decreased during corneal healing after RGTA treatment faster than after placebo application. Following the injury with the high alkali concentration, corneal inflammation and neo-vascularization were highly pronounced in placebo-treated corneas, whereas in RGTA-treated corneas they were significantly supressed. When RGTA or placebo application was started later after alkali injury and corneas were ulcerated, subsequent RGTA treatment healed the majority of them. In conclusion, RGTA facilitates the healing of injured corneas via a reduction of proteolytic, oxidative and nitrosative damage. Histol Histopathol 29, 457-478 (2014)es
dc.description.sponsorshipHolanes
dc.formatapplication/pdfes
dc.format.extent22es
dc.identifier.citationHistology and Histopathology, vol. 29, nº 4, (2014)
dc.identifier.issn1699-5848
dc.identifier.issn0213-3911
dc.identifier.urihttp://hdl.handle.net/10201/67919
dc.languageenges
dc.publisherF. Hernández y Juan F. Madrid. Universidad de Murcia. Departamento de Biología Celular e Histologíaes
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.subjectAlkali-injured corneaes
dc.subjectRGTA treatmentes
dc.subject.otherCDU::6 - Ciencias aplicadas::61 - Medicinaes
dc.titleThe healing of alkali-injured cornea is stimulated by a novel matrix regenerating agent (RGTA, CACICOL20) - a biopolymer mimicking heparan sulfates reducing proteolytic, oxidative and nitrosative damagees
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
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Histology and histopathology Vol.29, nº 4 (2014)