Long-term effect of optic nerve axotomy on the retinal ganglion cell layer

Nadal-Nicolás, Francisco Manuel; Sobrado Calvo, Paloma; Jiménez López, Manuel; Vidal Sanz, Manuel; Agudo Barriuso, Marta

Publication:
Long-term effect of optic nerve axotomy on the retinal ganglion cell layer

dc.contributor.author	Nadal-Nicolás, Francisco Manuel
dc.contributor.author	Sobrado Calvo, Paloma
dc.contributor.author	Jiménez López, Manuel
dc.contributor.author	Vidal Sanz, Manuel
dc.contributor.author	Agudo Barriuso, Marta
dc.contributor.department	Oftalmología, Optometría, Otorrinolaringología y Anatomía Patológica
dc.contributor.other	Facultades de la UMU::Facultad de Medicina
dc.date.accessioned	2026-01-27T08:41:05Z
dc.date.available	2026-01-27T08:41:05Z
dc.date.copyright	© 2015 The Association for Research in Vision and Ophthalmology, Inc.
dc.date.issued	2015-09-01
dc.description.abstract	Purpose: To analyze the long-term effect of optic nerve injury on retinal ganglion cells (RGCs) and melanopsin+RGCs orthotopic and displaced, and on the rest of the ganglion cell layer (GCL) cells. Methods: In adult albino rats, the left optic nerve was crushed (ONC) or transected (ONT). Injured and contralateral retinas were analyzed at increasing survival intervals (up to 15 months). To study all GCL cells and RGCs, retinas were immunodetected with Brn3a and melanopsin to identify the general RGC population (Brn3a+) and m+RGCs, and counter-stained with 4′,6-diamidino-2-phenylindole (DAPI). Brn3a+RGCs and m+RGCs displaced to the inner nuclear layer were analyzed as well. In additional retinas, glial cells in the GCL were identified with glial fibrillary acidic protein (GFAP) or Iba1, and in some retinas, Brn3a, calretinin, and γ-synuclein were immunodetected. Results: Orthotopic and displaced RGCs behave similarly within the RGC and m+RGC populations. Both lesions cause an exponential loss of RGCs (4%–1% survival at 6 months after ONC or ONT), but not of m+RGCs, whose number remains stable from 1 to 15 months (34%–44% of the initial population). γ-synuclein is expressed by RGCs and displaced amacrine cells (dACs), allowing us to confirm that axotomy does not affect the latter, and to determine that out of the approximately 217,406 cells that compose the GCL (excluding endothelia), 10% are glial cells, 50% dACs, and the remaining 40% are RGCs. Conclusions: In the GCL, only RGCs are lost after axotomy, and there are important differences in the course of loss and rate of survival between melanopsin+RGCs and the rest of RGCs.
dc.format	application/pdf
dc.identifier.citation	Invest Ophthalmol Vis Sci. 2015 Sep;56(10):6095-112.
dc.identifier.doi	https://doi.org/10.1167/iovs.15-17195
dc.identifier.eissn	1552-5783
dc.identifier.issn	0146-0404
dc.identifier.uri	http://hdl.handle.net/10201/194209
dc.language	eng
dc.publisher	Association for Research in Vision and Ophthalmology.
dc.relation	Supported by grants from the Spanish Ministry of Economy and Competitiveness: ISCIII-FEDER ‘‘Una manera de hacer Europa’’ PI13/00643; SAF-2012-38328 (Madrid, Spain)
dc.relation.publisherversion	https://iovs.arvojournals.org/article.aspx?articleid=2443691
dc.rights	Attribution 4.0 International	*
dc.rights.accessRights	info:eu-repo/semantics/openAccess
dc.rights.uri	http://creativecommons.org/licenses/by/4.0/	*
dc.subject	Intrinsically photosensitive retinal ganglion cells
dc.subject	Topography
dc.subject	Brn3a
dc.subject	Melanopsin
dc.subject	Displaced amacrine cells
dc.subject	Gamma synuclein
dc.subject	Displaced retinal ganglion cells
dc.subject.ods	Objetivo 3: Salud
dc.title	Long-term effect of optic nerve axotomy on the retinal ganglion cell layer
dc.type	info:eu-repo/semantics/article
dc.type.version	info:eu-repo/semantics/publishedVersion
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