Publication: C6orf15 expression in thyroid tumors: A promising diagnostic biomarker for the classic variant of papillary thyroid carcinoma
Authors
Pengju Zhang ; Jiaxin Chai ; Jiandong Wang ; Pengfei Huang ; Xudong Gu ; Aobo Xu
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Publisher
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Universidad de Murcia, Departamento de Biologia Celular e Histiologia
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DOI
https://doi.org/10.14670/HH-25-007
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info:eu-repo/semantics/article
Description
Abstract
Although C6orf15 is highly expressed in
certain human cancers, its expression pattern in papillary
tumors remains unclear. In this study, we investigated
C6orf15 expression in papillary tumors and assessed its
potential as a diagnostic biomarker for histopathological
evaluation and fine-needle aspiration cytology (FNAC).
We collected a total of 87 formalin-fixed and paraffin
embedded (FFPE) thyroid tissue specimens that
included: 10 cases with Hashimoto's thyroiditis (HT), 11
with follicular adenomas (FAs), two with non-invasive
follicular thyroid neoplasms with papillary-like nuclear
feature (NIFTP), six with follicular thyroid carcinomas
(FTCs), three with invasive encapsulated follicular
variant of papillary thyroid carcinomas (IEFVPTCs),
three with medullary thyroid carcinomas (MTCs), and
52 with papillary thyroid carcinomas (PTCs).
Additionally, 33 FNAC samples from thyroid nodules
were analyzed, comprising three samples of FA, five
atypia of undetermined significance (AUS), and 25 cases
of PTC. Immunohistochemical (IHC) staining was
performed to assess C6orf15 expression in thyroid tumor
tissues and FNAC samples. We conducted BRAF V600E
mutation analysis via Sanger sequencing and IHC and
discerned that C6orf15 expression was absent in normal
follicular epithelial cells, FA, NIFTP, TFC, and MTC.
The positivity rates for C6orf15 in FFPE samples were
as follows: 66.7% for IEFVPTC, 86.5% for PTC, and 60.0% for AUS. In FNAC samples, the positivity rate
was 80.0% for PTC. A significant positive correlation
was observed between C6orf15 expression and the
BRAF V600E mutation in PTC tissues (p<0.001), but no
such association was found in FNAC samples (p=0.230).
C6orf15 exhibited high expression levels in the majority
of IEFVPTC (66.7%), PTC tissues (86.5%), and FNAC
samples (80.0%). These findings suggest that C6orf15
constitutes a promising diagnostic biomarker for the
classic variant of PTC and is applicable to histo
pathological assessment and FNAC-based diagnosis.
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