Publication: Immunohistochemical study of serum albumin in normal and cadmium-treated mouse testis organs by “in vivo cryotechnique”
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Date
2006
Authors
Liao, X. ; Terada, N. ; Ohno, N. ; Li, Z. ; Fuji, Y. ; Baba, T. ; Ohno, S.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
The in vivo injection of cadmium (Cd) was
reported to induce blood-testis barrier disruption, and
assumed to be an experimental model to examine
junctional structures in seminiferous tubules. The
purpose of this study is to investigate time-dependent
changes of albumin permeability in the normal or Cdtreated
mouse testis by our “in vivo cryotechnique” with
immunohistochemistry, reflecting tight junctional (TJ)
barriers of Sertoli cells. The albumin in the seminiferous
tubules was firstly immobilized by the cryotechnique, in
which normal blood circulation was always kept. The
cryofixed testicular tissues were then processed for
freeze-substitution, and embedded in the paraffin wax.
Serial sections were immunostained by anti-mouse
albumin antibody with peroxidase immunostaining, and
also stained with hematoxylin-eosine (HE) for
morphological observation. In normal seminiferous
tubules, the immunoreaction products were localized
around peritubular myoid cells and between Leydig
cells, as well as in blood vessels. They were also
localized as arch-like patterns around some
spermatogonia in basal compartments of seminiferous
tubules. Twenty-four and 48 hrs after Cd-treatment,
some enlarged spaces and vesicular formations in the
seminiferous epithelium were observed on the HEstained
sections. The albumin immunolocalization was
detected not only in the basal compartments, but also in
the adluminal compartments between Sertoli cells and
germ cells. Thus, the structural disruptions of inter-
Sertoli TJ barriers could be clearly demonstrated by the
“in vivo cryotechnique”.
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