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Isolation of functional mature peritoneal macrophages from healthy humans

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dc.contributor.authorRuiz Alcaraz, Antonio José
dc.contributor.authorMartınez-Banaclocha, Helios
dc.contributor.authorMarín Sánchez, Pilar
dc.contributor.authorCarmona-Martínez Violeta
dc.contributor.authorIniesta-Albadalejo, Miguel Angel
dc.contributor.authorTristan-Manzano, María
dc.contributor.authorGarcía Peñarrubia, María del Pilar
dc.contributor.authorMachado Linde, Francisco
dc.contributor.authorPelegrín Vivancos, Pablo
dc.contributor.authorMartínez-Esparza Alvargonzález, María Concepción
dc.contributor.authorTapia Abellán, Ana
dc.contributor.departmentBioquímica y Biología Molecular B e Inmunología
dc.contributor.otherFacultad de Biología
dc.date.accessioned2026-01-12T12:29:46Z
dc.date.available2026-01-12T12:29:46Z
dc.date.copyright© 2019 Australian and New Zealand Society for Immunology Inc.
dc.date.issued2019-11-11
dc.description.abstractMacrophages play an important role in the inflammatory response. Their various biological functions are induced by different membrane receptors, including Toll-like receptors, which trigger several intracellular signaling cascades and activate the inflammasomes, which in turn elicit the release of inflammatory mediators such as cytokines. In this study, we present a novel method for the isolation of human mature peritoneal macrophages. This method can be easily implemented by gynecologists who routinely perform laparoscopy for sterilization by tubal ligation or surgically intervene in benign gynecological pathologies. Our method confirms that macrophages are the main peritoneal leukocyte subpopulation isolated from the human peritoneum in homeostasis. We showed that primary human peritoneal macrophages present phagocytic and oxidative activities, and respond to activation of the main proinflammatory pathways such as Toll-like receptors and inflammasomes, resulting in the secretion of different proinflammatory cytokines. Therefore, this method provides a useful tool for characterizing primary human macrophages as control cells for studies of molecular inflammatory pathways in steady-state conditions and for comparing them with those obtained from pathologies involving the peritoneal cavity. Furthermore, it will facilitate advances in the screening of anti-inflammatory compounds in the human system.
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dc.format.extent13
dc.identifier.citationImmunology & Cell Biology 2020; 98: 114–126
dc.identifier.doihttps://doi.org/10.1111/imcb.12305
dc.identifier.eissn1440-1711
dc.identifier.issn0818-9641
dc.identifier.urihttp://hdl.handle.net/10201/185789
dc.languageeng
dc.publisherWiley
dc.relationSupport through the Precipita crowdfunding platform (PR200; Endometriosis), especially Macarena Guillamon, Mercedes Jimenez, Ramon Diaz Petrel, Victoria Millo and Lucıa Arnaiz (Endomeque). We also want to acknowledge the individuals who participated in this study and thank the BioBank Biobanco en Red de la Region de Murcia (PT13/0010/0018) integrated into the Spanish National Biobanks Network (B.000859) for its collaboration. The study was supported by the Precipita crowdfunding platform (PR200; Endometriosis to PG-P), Fundacion Espa~nola para la Ciencia y la Tecnologıa Ministerio de Ciencia y Universidades, Spain, the Ministerio de Economıa, Industria y Competitividad (SAF2017-88276-R to PP), the Fundacion Seneca (20859/PI/18 to PP) and the European Research Council (ERC-2013-CoG 614578 to PP). HM-B was supported by a Rio Hortega fellowship from the Instituto Salud Carlos III (CM14/00008). VC-M was supported by the Direccion General de Investigacion Cientıfica y Tecnica (EJ-2014-A-95803), Spain.
dc.relation.publisherversionhttps://onlinelibrary.wiley.com/doi/10.1111/imcb.12305
dc.rights.accessRightsinfo:eu-repo/semantics/restrictedAccess
dc.subjectHuman macrophages
dc.subjectInflammation
dc.subjectInnate immunity
dc.subjectPeritoneal leukocytes
dc.subjectCytokines
dc.subject.odsNo relacionado con ningún objetivo de desarrollo sostenible
dc.titleIsolation of functional mature peritoneal macrophages from healthy humans
dc.typeinfo:eu-repo/semantics/article
dc.type.versioninfo:eu-repo/semantics/publishedVersion
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