Publication: A qualitative comparison of ten tissue clearing techniques
Authors
Orlich, Michael ; Kiefer, Friedemann
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Publisher
Universidad de Murcia. Departamento de BiologĂa Celular e HistologĂa
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DOI
DOI: 10.14670/HH-11-903
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info:eu-repo/semantics/article
Description
Abstract
The understanding of spatially complex
biological systems is greatly aided by the availability of
high resolution information on their tissue architecture,
as is provided by optical sectioning microscopy like
confocal and light sheet microscopy. In addition,
genetically encoded fluorescent reporter proteins reveal
tissue architecture without the need for staining
procedures. Owing to opacity caused by scattering and
absorption, light microscopy in tissue is limited to thin
tissue layers of a few micrometers traditionally provided
by histological sections. Aiming to allow deeper
imaging, during the last decade massive efforts to
develop tissue clearing protocols produced a flurry of
novel clearing techniques for whole organ visualization,
now available to microscopists. In particular, new tissue
clearing methods were developed that avoid the use of
organic solvents, aiming to retain the integrity of
genetically encoded fluorescent proteins. So far, these
methods have not been directly compared and selection
of the right technique can be a non-trivial task. Here, we
have aimed to compare different tissue clearing
approaches side by side in a standardized manner. We
provide qualitative data on their clearing capability of
mouse brain, lung, heart, kidney and muscle, as well as
embryos and fetuses at the developmental stages E10.5,
E12.5 and E15.5 and discuss possible applications.
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Citation
Histology and Histopathology, Vol.33, nÂş2, (2018)
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