Publication: Evaluation of the multiple tissue factors in bone of primary osteoplasty and rhinoplasty in patients affected by cleft lip palate
Authors
Buile, Dace ; Pilmane, Māra ; Akota, Ilze
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Publisher
Universidad de Murcia, Departamento de Biologia Celular e Histiologia
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DOI
https://doi.org/10.14670/HH-18-451
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info:eu-repo/semantics/article
Description
Abstract
Clefts of the lip and/or palate (CLP) are
visible disruptions of standard facial structure. The aim
of our study was to determine a relative number and
appearance of the tissue factors in bone of patients with
CLP during first time plastic alveolar osteoplasty or
rhinoplasty.
Immunohistochemistry was performed with matrix
metalloproteinase-8 (MMP-8), matrix metalloproteinase9 (MMP-9), osteopontin (OPN), osteocalcin (OC), Runtrelated transcription factor 2 (Runx2), beta-defensin-2
(βdef-2), beta-defensin-3 (βdef-3), interleukin-1 alpha
(IL-1α), and interleukin-10 (IL-10). The bone formation
was observed by Masson-trichrome (Masson) staining.
For the quantification of structures, the semi-quantitative
census method was used. Spearman rank order
correlation coefficient and Mann-Whitney U test were
used for the statistical analysis.
A significantly higher number of OPN positive
osteocytes was observed in the CLP group when
compared to the control group (p=0.002). The number of
OC positive osteocytes (p=0.000) and βdef-2 positive
osteocytes (p=0.003) was significantly lower in the CLP
group in comparison to the control group. Strong,
positive correlations between IL-10 and OC (rs=0.608;
p=0.002), IL-1α and MMP-9 (rs=0.666; p=0.000), OPN
and MMP-8 (rs=0.620; p=0.002) were detected in the
CLP group. A tendency for the increased appearance of
MMP-8, MMP-9 positive osteocytes of the patients with
CLP, suggests elevated tissue remodelling properties.
Increased appearance of OPN positive osteocytes in
bone of the patients with CLP shows increased bone
homeostasis based on seriously decreased
mineralization, which may be a possible compensatory
reaction to decreased quality of postsurgical bone.
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Citation
Histology and Histopathology Vol. 37, nº7 (2022)
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