Publication: Valproic acid during pregnancy decrease the number of spermatogenic cells and testicular volume in the offspring of mice: Stereological quantification
Authors
Conei, Daniel ; Rojas, Mariana ; SantamarÃa, Luis ; Risopatrón, Jennie
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Publisher
Universidad de Murcia, Departamento de Biologia Celular e Histiologia
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DOI
https://doi.org/10.14670/HH-18-380
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info:eu-repo/semantics/article
Description
Abstract
Valproic acid (VPA) is a drug used to treat
epilepsy, bipolar disorders and headaches. As a
secondary effect, this antiepileptic drug can cause a
decrease in androgens and gonadotropins, and dosedependent testicular defects, such as reduction of
testicular weights, sperm motility and degeneration of
the seminiferous tubules. In offspring exposed to VPA,
its effects have not been evaluated, so the study aimed to
determine the morphological effects of the use of VPA
along testicular development in mice. 30 adult female
BALB/c mice were crossed and divided by age, with
embryos of 12.5 days post coitum (dpc), fetuses of 17.5
dpc and male mice 6 weeks postnatal. In each case, the
pregnant mouse received 600 mg/kg of VPA, making up
the VPA groups, or 0.3 mL of 0.9% physiological
solution for the control groups, from the beginning to the
end of the pregnancy, orally.t. A morpho-quantitative
analysis was carried out on the gonadal development of
the male offspring. In the groups treated with VPA, at all
ages studied they had lower testicular volume. At 12.5
dpc, they showed less testicular development in the form
of sex cords, with fewer gonocytes and somatic cells. At
17.5 dpc, they presented greater interstitial space, fewer
spermatogonial, sustentacular Sertoli, peritubular and
interstitial Leydig cells. At 6 weeks postnatal, they
presented fewer spermatogonia, pachytene
spermatocytes, elongated spermatids, sustentacular
Sertoli and interstitial Leydig cells, with statistically
significant differences. In conclusion, prenatal exposure
to VPA causes histopathological alterations in the
offspring of mice in testicular development, from the
embryonic stage to 6 weeks postnatal.
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Citation
Histology and Histopathology Vol. 36, nº11 (2021)
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