Effect of a dirhamnolipid biosurfactant on the structure and phase behaviour of dimyristoylphosphatidylserine model membranes

Oliva, Alfonso; Aranda Martínez, Francisco José; Ortiz López, Antonio; Teruel Puche, José Antonio

Publication:
Effect of a dirhamnolipid biosurfactant on the structure and phase behaviour of dimyristoylphosphatidylserine model membranes

dc.contributor.author	Oliva, Alfonso
dc.contributor.author	Aranda Martínez, Francisco José
dc.contributor.author	Ortiz López, Antonio
dc.contributor.author	Teruel Puche, José Antonio
dc.contributor.department	Bioquímica y Biología Molecular A	es
dc.date.accessioned	2024-12-16T13:00:42Z
dc.date.available	2024-12-16T13:00:42Z
dc.date.issued	2019-10-19
dc.description.abstract	Rhamnolipids are bacterial biosurfactants containing one or two rhamnose rings and a hydrophobic hydrocarbon portion. These compounds are mainly isolated from Pseudomonas spp culture media, and have been shown to present outstanding biological activities. A number of experimental works have shown that the interaction of rhamnolipids with target membranes could play a role in these actions. Therefore the study of the interaction of purified rhamnolipids with the various phospholipid components of biological membranes is of great interest. This paper shows the phase behaviour of mixtures of 1,2-dimyristolylphosphatidylserine (DMPS) with a dir hamnolipid (diRL) fraction produced by P. aeruginosa. This experimental approach has been based on the use of physical techniques such as Differential Scanning Calorimetry (DSC) and Fourier-Transform Infrared Spectroscopy (FTIR). DSC indicated that the presence of increasing concentrations of diRL in the bilayer resulted in a progressive broadening of the gel to liquid-crystalline phase transition of DMPS. In addition a complex thermal behaviour was observed, with the presence of more than one transition at higher concentrations of the biosurfactant, indicating phase separation. FTIR showed that diRL increased the proportion of gauche rotamers of DMPS, thus affecting acyl chain order. The change in the frequency of the carboxylate stretching band of DMPS observed upon interaction with diRL pointed toward changes in the local environment of the polar headgroup of the phospholipid, resulting in a modification of its conformation or insertion within the bilayer. This result was corroborated by the effect of diRL on the carbonyl and phosphate stretching bands of DMPS, showing an increase of the hydration both in the gel and in the liquid-crystalline phase. Molecular Dynamics (MD) simulations gave further support to the experimental results, showing diRL cluster formation as well as an augmented exposition of DMPS to the water layer in the presence of the biosurfactant.	es
dc.format	application/pdf	es
dc.format.extent	8	es
dc.identifier.citation	Colloids and Surfaces B: Biointerfaces. 185 (2020) 110576
dc.identifier.doi	https://doi.org/10.1016/j.colsurfb.2019.110576
dc.identifier.issn	1873-4367
dc.identifier.issn	0927-7765
dc.identifier.uri	http://hdl.handle.net/10201/147513
dc.language	eng	es
dc.relation	Sin financiación externa a la Universidad	es
dc.relation.publisherversion	Elsevier	es
dc.rights.accessRights	info:eu-repo/semantics/restrictedAccess
dc.subject	Rhamnolipids	es
dc.subject	Biosurfactants	es
dc.subject	Phospholipid membranes	es
dc.subject	Phosphatidylserine	es
dc.subject	Pseudomonas aeruginosa	es
dc.title	Effect of a dirhamnolipid biosurfactant on the structure and phase behaviour of dimyristoylphosphatidylserine model membranes	es
dc.type	info:eu-repo/semantics/article	es
dspace.entity.type	Publication	es
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