Publication: NPY and TH innervation in human choroidal whole-mounts
Authors
Triviño, A. ; Hoz, R.de ; Rojas, Blanca ; Salazar, J.J. ; Ramírez, Ana I. ; Ramírez, José M.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
To determine the distribution of NPY and TH
human choroidal innervation, choroidal whole-mounts
were processed for indirect immunofluorescence. An
antibody to a component of the neuronal cytoskeleton,
neurofilament 200 kDa (NF-200) was used to identify
neurons and axons. A double immunostaining was
performed, antibodies against NF-200 being combined
with antibodies against neuropeptide Y (NPY) and
tyroxine hydroxylase (TH). Fibers containing both NPY
and TH were distributed in three plexuses, one in the
suprachoroid large-sized vessel layer, and two in the
medium-sized vessel layer. Intrinsic choroidal neurons
(ICNs) containing NPY and TH were observed in the
suprachoroid. The TH(+) ICNs were located in the
medium-sized vessel layer. Overall, NPY(+) and TH(+)
ICNs were more frequent in the central temporal area,
both in isolation and forming microganglia. We also
detected small spindle elements intensely
immunoreactive to TH(+) and distributed mainly in the
suprachoroid from the equator to the periphery. In
conclusion, the human choroid contains abundant NPY
and TH nerve fibers related to chroroidal vascular
structures; it further possesses NPY(+) and TH(+) ICNs
which contribute to the choroidal self-regulation
persisting after sympathetic denervation. Additionally,
these ICNs may at least partially explain why the
choroidal blood flow does not respond to the factors that influence systemic vascular control. The preferential
location of these cells in the submacular area suggests
that dysfunction or degeneration of these cells may be a
factor in vascular pathologies found in ocular disease,
such as diabetic macular edema or age-related macular
degeneration.
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