Publication: Spontaneous and ART-induced large offspring syndrome: similarities and differences in DNA methylome
Authors
Yahan Li ; Sena Lopes, Jordana ; Coy-Fuster, Pilar ; Melissa Rivera, RocĂo
item.page.secondaryauthor
item.page.director
Publisher
Taylor and Francis Group
publication.page.editor
publication.page.department
DOI
https://doi.org/10.1080/15592294.2022.2067938
item.page.type
info:eu-repo/semantics/article
Description
© 2022 The Author(s)____ This document is the published version of a published work that appeared in final form in Epigenetics____
This document is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0 ____
To access the final edited and published work see:
https://doi.org/10.1080/15592294.2022.2067938
Abstract
Large/abnormal offspring syndrome (LOS/AOS) is a congenital overgrowth syndrome reported in ruminants produced by assisted reproduction (ART-LOS) which exhibit global disruption of the epigenome and transcriptome. LOS/AOS shares phenotypes and epigenotypes with the human congenital overgrowth condition Beckwith-Wiedemann syndrome. We have reported that LOS occurs spontaneously (SLOS); however, to date, no study has been conducted to determine if SLOS has the same methylome epimutations as ART-LOS. In this study, we performed whole- genome bisulphite sequencing to examine global DNA methylation in bovine SLOS and ART-LOS tissues. We observed unique patterns of global distribution of differentially methylated regions (DMRs) over different genomic contexts, such as promoters, CpG Islands, shores and shelves, as well as at repetitive sequences. In addition, we included data from two previous LOS studies to identify shared vulnerable genomic loci in LOS. Overall, we identified 320 genomic loci in LOS that have alterations in DNA methylation when compared to controls. Specifically, there are 25 highly vulnerable loci that could potentially serve as molecular markers for the diagnosis of LOS, including at the promoters of DMRT2 and TBX18, at the imprinted gene bodies of IGF2R, PRDM8, and BLCAP/NNAT, and at multiple CpG Islands. We also observed tissue-specific DNA methylation patterns between muscle and blood, and conservation of ART-induced DNA methyla-tion changes between muscle and blood. We conclude that as ART-LOS, SLOS is an epigenetic condition. In addition, SLOS and ART-LOS share similarities in methylome epimutations.
publication.page.subject
Citation
Epigenetics 2022, VOL. 17, NO. 11, 1477–149
item.page.embargo
Collections
Ir a EstadĂsticas
Este Ătem está sujeto a una licencia Creative Commons. http://creativecommons.org/licenses/by-nc-nd/4.0/