Publication: Histological and histochemical evaluation of human oral mucosa constructs developed by tissue engineering
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Date
2007
Authors
Sánchez-Quevedo, M.C. ; Alaminos, M. ; Capitan, L.M. ; Moreu, G. ; Garzon, I. ; Crespo, P.V. ; Campos, Antonio
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
Reconstruction of large oral mucosa defects
is often challenging, since the shortage of healthy oral
mucosa to replace the excised tissues is very common. In
this context, tissue engineering techniques may provide a
source of autologous tissues available for transplant in
these patients. In this work, we developed a new model
of artificial oral mucosa generated by tissue engineering
using a fibrin-agarose scaffold. For that purpose, we
generated primary cultures of human oral mucosa
fibroblasts and keratinocytes from small biopsies of
normal oral mucosa using enzymatic treatments. Then
we determined the viability of the cultured cells by
electron probe quantitative X-ray microanalysis, and we
demonstrated that most of the cells in the primary
cultures were alive and had high K/Na ratios. Once cell
viability was determined, we used the cultured
fibroblasts and keratinocytes to develop an artificial oral
mucosa construct by using a fibrin-agarose extracellular
matrix and a sequential culture technique using porous
culture inserts. Histological analysis of the artificial
tissues showed high similarities with normal oral
mucosa controls. The epithelium of the oral substitutes
had several layers, with desmosomes and apical
microvilli and microplicae. Both the controls and the expression of cytokeratin 13 and low expression of
cytokeratin 10. All these results suggest that our model
of oral mucosa using fibrin-agarose scaffolds show
several similarities with native human oral mucosa.
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