Publication: The expression of the epitope H recognized by the monoclonal antibody H is higher in astrocytomas
compared to anaplastic astrocytomas and glioblastomas
Authors
Arvanitis, D. ; Arvanitis, L. ; Panourias, I.G. ; Kitsoulis, P. ; Kanavaros, Panagiotis
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
The epitope H contains an O-linked Nacetylglucosamine
residue in a specific conformation
and/or environment recognized by the monoclonal
antibody H (mAbH). mAbH stains two bands with Mr
x10-3 of 209 and 62 in lysates of cultured rat astrocytes.
In normal human brains epitope H is absent from the
overwhelming majority of normal astrocytes and only
sparse reactivity is observed, confined mostly to fibrous
astrocytes. Upregulation of the epitope H takes place in
reactive astrocytes. In the present study we used the
mAbH to investigate the immunohistochemical
expression of the epitope H in 41 cases of astrocytic
tumors including 19 cases of astrocytomas, 8 cases of
anaplastic astrocytomas and 14 cases of glioblastomas.
Seven out of 19 cases (37%) of astrocytomas showed
weak staining, 10 cases (53%) moderate staining and 2
cases (10%) intense staining. Two out of 8 cases (25%)
of anaplastic astrocytomas appeared negative, 3 cases
(37.5%) showed weak staining and 3 cases (37.5%)
moderate staining. Four out of 14 cases (28.5) of glioblastomas appeared negative, 7 cases (50%) showed
weak staining, 2 cases (14%) showed moderate staining
and only one case (7.5%) showed intense staining. There
was a statistically significant elevation of the expression
of the epitope H in astrocytomas compared to anaplastic
astrocytomas and glioblastomas (p=0.047). These results
indicate that the expression of the epitope H decreases in
parallel with the increase of the grade of astrocytic
tumors from low to higher grade neoplasms. This could
be of interest for predicting the progression of an
astrocytic tumor since it is documented that
astrocytomas progress to tumors of higher grade of
malignancy. Further investigation of the antigens bearing
the epitope H might help to gain further insight into the
mechanisms which regulate the progression of astrocytic tumors and to examine the relevance of the mAbH
staining with respect to the prognosis of these
neoplasms.
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