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In vivo confocal microscopy assessment of the corneoscleral limbal stem cell niche before and after biopsy for cultivated limbal epithelial transplantation to restore corneal epithelium

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dc.contributor.authorRamírez, Beatriz E.
dc.contributor.authorVictoria, Darío A.
dc.contributor.authorMurillo, Giovana M.
dc.contributor.authorHerreras, José M.
dc.contributor.authorCalonge, Margarita
dc.date.accessioned2020-02-11T19:35:52Z
dc.date.available2020-02-11T19:35:52Z
dc.date.issued2015
dc.description.abstractAutologous cultivated limbal epithelial transplantation (CLET) is a successful therapy to restore corneal epithelium when limbal epithelial stem cells are damaged unilaterally, which can result in corneal blindness. We used in vivo confocal microscopy (IVCM) to identify the best location in the corneoscleral limbal niche and to harvest autologous epithelial stem cells for CLET. We also ascertained the completeness of limbal structure removal after biopsy and followed the healing process for any evidence of limbal structure reappearance. The 4 meridians of the corneoescleral limbus of 5 healthy donor eyes were scanned clinically and by IVCM before biopsy and 1 week, 1, 3, and 6 months after. IVCM detected palisades of Vogt, the limbal niche hallmark, more efficiently (100%) than clinically (60%), and were consistently better defined at the 12 o’clock meridian, and so this was the site selected for biopsy. The depth of palisades was 80.4±19.8 µm, and of the limbal biopsies was 136.8±19.1 µm, thus assuring that the limbal niche was completely harvested in all cases. Re-epithelialization of the donor site was complete at 1 week. The limbal wound was refilled with fibrovascular tissue, and no limbal-like structures reappeared. The study shows that clinical absence of palisades of Vogt is not necessarily an exclusionary criterion for autologous CLET. IVCM was useful to select the best place for limbal biopsy and identified features not visible clinically. IVCM also confirmed complete removal of limbal tissue by the biopsy. Limbal niche structures did not reappear by 6 months after surgery.es
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dc.format.extent10es
dc.identifier.citationHistology and Histopathology, Vol. 30, n.º 2 (2015)
dc.identifier.doihttps://doi.org/10.14670/HH-30.183
dc.identifier.issn1699-5848
dc.identifier.issn0213-3911
dc.identifier.urihttp://hdl.handle.net/10201/86545
dc.languageenges
dc.publisherF. Hernández y Juan F. Madrid. Universidad de Murcia: Departamento de Biología Celular e Histologíaes
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectPalisades of Vogtes
dc.subjectLimbal biopsyes
dc.subjectIn vivo confocal microscopyes
dc.subject.otherCDU::5 - Ciencias puras y naturales::57 - Biología::576 - Biología celular y subcelular. Citologíaes
dc.titleIn vivo confocal microscopy assessment of the corneoscleral limbal stem cell niche before and after biopsy for cultivated limbal epithelial transplantation to restore corneal epitheliumes
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
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Histology and histopathology Vol.30, nº2 (2015)