Publication: Cultured macrophages cause dissolucytosis of metallic silver
Authors
Jansons Locht, Linda ; Larsen, Agnete ; Stoltenberg, Meredin ; Danscher, Gorm
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
The present study proves that cultured
macrophages can liberate silver ions from metallic silver
surfaces by a process called dissolucytosis. Macrophages
(J774) were grown on a silver plate for different periods
of time and after fixation in glutaraldehyde, they were
subjected to autometallograhy in order to amplify
possible cellular silver-sulphur nanocrystals. Light and
electron microscopic analysis of the cells revealed that
silver ions released from the plate had been taken up by
the macrophages and accumulated in lysosome- like
structures.
We found that the liberation of silver ions takes
place extracellularly and is caused by chemical activity
in a dissolution membrane, most likely secreted and
organized by the macrophages. The liberation and the
subsequent uptake of silver ions in the macrophages is a
relatively fast process and the resulting silver-sulphur
nanocrystals can be observed in macrophages that have
been in contact with metallic silver for only a few
minutes. Our findings indicate that the speed of
dissolucytosis is highly influenced by the chemical
nature of the object exposed to the dissolucytotic process
which is likely to occur whenever macrophages
encounter a non-phagocytosable foreign object.
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