Publication: Ultrastructural study of upper surface layer in rat
mandibular condylar cartilage by quick-freezing method
Authors
Yoshida, M. ; Zea-Aragón, Z. ; Ohtsuki, K. ; Ohnishi, M. ; Ohno, S.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
The purpose of the present study is to clarify
native ultrastructures of upper surface layers of the rat
mandibular condylar cartilage in vivo by a quickfreezing
method. The mandibular cartilaginous tissues
were removed with their articular discs attached without
opening the lower joint cavity. The specimens were
processed for light microscopy, transmission or scanning
electron microscopy. Deep-etching replica membranes
were also prepared after the routine quick-freezing
method. The upper surface layer was well preserved by
the quick-freezing method. The cartilaginous tissues,
which were fixed without opening their articular discs,
appeared to keep better morphology than those after
opening them. The upper surface layer was thicker than
the corresponding layer as reported before. It consisted
of atypical extracellular matrices with lots of apparently
amorphous components, which were distributed over
typical collagen fibrils, by conventional electron
microscopy. As revealed with the replica membranes, it
also consisted of variously sized filaments and tiny
granular components localized on the typical collagen
fibrils. A pair of stereo-replica electron micrographs
three-dimensionally showed compact filaments within
the upper surface layer. The quick-freezing method was
useful for keeping native ultrastructures of the fragile
upper surface layer in the mandibular condylar cartilage,
which may be functionally important to facilitate smooth
movement of the temporomandibular joint.
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