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Control of morphology and virulence by ADP-ribosylation factors (Arf) in Mucor circinelloides

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dc.contributor.authorPatiño-Medina, Jose Alberto
dc.contributor.authorMaldonado-Herrera, Guadalupe
dc.contributor.authorPérez Arques, Carlos
dc.contributor.authorAlejandre-Castañeda, Viridiana
dc.contributor.authorReyes-Mares, Nancy Yadira
dc.contributor.authorValle-Maldonado, Marco I.
dc.contributor.authorCampos-García, Jesus
dc.contributor.authorOrtiz-Alvarado, Rafael
dc.contributor.authorJácome-Galarza, Irvin E.
dc.contributor.authorRamírez-Díaz, Martha I.
dc.contributor.authorGarre Mula, Victoriano
dc.contributor.authorMeza-Carmen, Victor
dc.contributor.departmentGenética y Microbiología
dc.contributor.otherFacultades de la UMU::Facultad de Biología
dc.date.accessioned2026-02-28T22:07:51Z
dc.date.available2026-02-28T22:07:51Z
dc.date.copyright© Springer-Verlag GmbH Germany, part of Springer Nature 2017.
dc.date.issued2017-12-20
dc.description.abstractMucor circinelloides is a dimorphic fungus used to study cell differentiation that has emerged as a model to characterize mucormycosis. In this work, we identified four ADP-ribosylation factor (Arf)-encoding genes (arf1-arf4) and study their role in the morphogenesis and virulence. Arfs are key regulators of the vesicular trafficking process and are associated with both growth and virulence in fungi. Arf1 and Arf2 share 96% identity and Arf3 and Arf4 share 89% identity, which suggests that the genes arose through gene-duplication events in M. circinelloides. Transcription analysis revealed that certain arf genes are affected by dimorphism of M. circinelloides, such as the arf2 transcript, which was accumulated during yeast development. Therefore, we created knockout mutants of four arf genes to evaluate their function in dimorphism and virulence. We found that both arf1 and arf2 are required for sporulation, but these genes also perform distinct functions; arf2 participates in yeast development, whereas arf1 is involved in aerobic growth. Conversely, arf3 and arf4 play only minor roles during aerobic growth. Moreover, we observed that all single arf-mutant strains are more virulent than the wild-type strain in mouse and nematode models, with the arf3 mutant being most virulent. Lastly, arf1/arf2 and arf3/arf4 double mutations produced heterokaryon strains that did not reach the homokaryotic state, indicating that these genes participate in essential and redundant functions. Overall, this work reveals that Arfs proteins regulate important cellular processes in M. circinelloides such as morphogenesis and virulence, laying the foundation to characterize the molecular networks underlying this regulation.
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dc.identifier.citationPatiño-Medina, J.A., Maldonado-Herrera, G., Pérez-Arques, C. et al. Control of morphology and virulence by ADP-ribosylation factors (Arf) in Mucor circinelloides . Curr Genet 64, 853–869 (2018). https://doi.org/10.1007/s00294-017-0798-0
dc.identifier.doihttps://doi.org/10.1007/s00294-017-0798-0
dc.identifier.eissn3091-3217
dc.identifier.eissn1432-0983
dc.identifier.urihttp://hdl.handle.net/10201/216442
dc.languageeng
dc.publisherSpringer Nature
dc.relation19339/PI/14. Fundación Séneca. Regional FPU14/01832. MECD. Nacional
dc.relation.publisherversionhttps://link.springer.com/article/10.1007/s00294-017-0798-0
dc.rightsAttribution 4.0 International*
dc.rights.accessRightsinfo:eu-repo/semantics/openAccess
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectADP-ribosylation factor
dc.subjectArf protein
dc.subjectFungal virulence
dc.subjectMucor
dc.subjectSecretion
dc.subjectVesicle trafficking
dc.subject.odsObjetivo 3: Salud
dc.titleControl of morphology and virulence by ADP-ribosylation factors (Arf) in Mucor circinelloides
dc.typeinfo:eu-repo/semantics/article
dc.type.versioninfo:eu-repo/semantics/publishedVersion
dspace.entity.typePublicationes
relation.isAuthorOfPublication6f9dff61-8e76-45ff-9d5e-3510ac6aa452
relation.isAuthorOfPublicationa0bda0ef-b067-4de7-8130-7c6886c400e3
relation.isAuthorOfPublication.latestForDiscovery6f9dff61-8e76-45ff-9d5e-3510ac6aa452
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