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A genetically encoded IL-1β BRET sensor to monitor inflammasome activity

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dc.contributor.authorCompan, Vincent
dc.contributor.authorBaroja-Mazo, Alberto
dc.contributor.authorBragg, Laricia
dc.contributor.authorVerkhratsky, Alexei
dc.contributor.authorPerroy, Julie
dc.contributor.authorPelegrín Vivancos, Pablo
dc.contributor.departmentBioquímica y Biología Molecular B e Inmunología
dc.date.accessioned2024-01-30T11:09:18Z
dc.date.available2024-01-30T11:09:18Z
dc.date.issued2012
dc.description© 2012 by The American Association of Immunologists, Inc.. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ This document is the Accepted, version of a Published Work that appeared in final form in Journal of Immunology. To access the final edited and published work see https://doi.org/10.4049/jimmunol.1201349
dc.description.abstractInflammation is fundamental for protecting the organism against infection and injury. However, a failure to control immune response results in chronic inflammation and several associated disorders such as pain and loss of function. Initiation of inflammation is orchestrated by cytokines, among which interleukin (IL)-1β is particularly important. IL- 1β is synthesized as an inactive protein that has to be processed by the inflammasome to generate the mature bioactive form. Conventional techniques cannot monitor IL-1β activation with high spatial and temporal resolution. Here, we present a ratiometric biosensor that allows monitoring IL-1β processing in real-time, with a temporal resolution of seconds and with a single cell spatial resolution. Using this sensor, we describe for the first time the kinetic of the inflammasome activity in living macrophages. With this new probe we also demonstrated that the pro-IL-1β processing occurs all over the cytoplasm.es
dc.formatapplication/pdfes
dc.format.extent39es
dc.identifier.citationJournal of Immunology, volumen 189, nº 5, año 2012, páginas 2131-2137
dc.identifier.doihttps://doi.org/10.4049/jimmunol.1201349
dc.identifier.urihttp://hdl.handle.net/10201/138145
dc.languageenges
dc.publisherAsociación Americana de Inmunologíaes
dc.relationWellcome Trust, Plan Nacional I+D+I 2008-2011- Instituto Salud Carlos III-FEDER (EMER07/049, PI09/0120), Fundación Séneca (11922/PI/09).es
dc.relation.publisherversionhttps://journals.aai.org/jimmunol/article/189/5/2131/39872/A-Genetically-Encoded-IL-1-Bioluminescencees
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International
dc.rights.accessRightsinfo:eu-repo/semantics/openAccess
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectInterleukin-1βes
dc.subjectMacrophagees
dc.subjectCaspase-1es
dc.subjectBRETes
dc.subject.otherCDU::6 - Ciencias aplicadases
dc.titleA genetically encoded IL-1β BRET sensor to monitor inflammasome activityes
dc.title.alternativeBiosensor for IL-1β imaginges
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
relation.isAuthorOfPublication547c4c2c-d906-4e1d-ae9a-0cdb40eef6d1
relation.isAuthorOfPublication.latestForDiscovery547c4c2c-d906-4e1d-ae9a-0cdb40eef6d1
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