Publication: Effect of coenzyme A on outer hair cells in cisplatin ototoxicity: functional and ultrastructural study
Authors
Fernández Cervilla, Francisco ; Martínez Martínez, María ; Fernández Segura, Eduardo ; Cañizares García, Francisco Javier ; Crespo Ferrer, Pascual Vicente
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Publisher
Universidad de Murcia. Departamento de Biología Celular e Histología
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DOI
DOI: 10.14670/HH-11-7751
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info:eu-repo/semantics/article
Description
Abstract
The aim of this study was to use functional
and morphological analyses to evaluate the protective
effect of coenzyme A (CoA) in cisplatin-induced toxicity
in outer hair cells (OHC). Three groups of 8 guinea pigs
were used: control (group I), cisplatin-treated (group II)
and cisplatin + CoA-treated (group III). In groups II and
III, a single ototoxic dose of cisplatin (10 mg/kg) was
injected intraperitoneally. Group III was co-treated with
CoA (900 μg/kg per day for 7 consecutive days).
Electrocochleography (ECoG) recordings were made
before and after the 7-day treatment period in all groups.
After ECoG on day 7, all animals were anesthetized and
the cochleae were removed and fixed for ultrastructural
analysis. Cell damage in OHC was observed with
transmission electron microscopy. Cisplatin induced a
significant increase in auditory thresholds (p<0.001)
compared to group I (control). In contrast, group III
(cisplatin + CoA) had significantly reduced thresholds
(p<0.001) compared to the group treated with cisplatin
alone (group II). We found no significant differences
between the control group and animals co-treated with
cisplatin and CoA. The electron microscopy findings in
OHC were consistent with these results. Ultrastructural
analysis of OHC in group II showed morphological
indications of necrosis, i.e. cytoplasmic swelling and
vacuolation, and mitochondrial swelling. In group III the
cell morphology of OHC was preserved, with ultrastructural characteristics similar to the control group. In
conclusion, co-treatment with cisplatin with CoA
inhibited antineoplastic-induced cytotoxicity in OHC in
a guinea pig model.
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Citation
Histology and Histopathology, Vol.32, nº2, (2017)
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