Publication: WuFu decoction alleviates osteoarthritis by regulating TGF-β1/Smad and ERK1/2 pathways
Authors
Linghua Zhou ; Jian Lin ; Pengzheng Yu ; Zhengcong Ye
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Publisher
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Universidad de Murcia, Departamento de Biologia Celular e Histiologia
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DOI
https://doi.org/10.14670/HH-25-017
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info:eu-repo/semantics/article
Description
Abstract
The age-standardized point prevalence of
global osteoarthritis (OA) has increased, and OA will not
only lead to disability in patients but also to a greater
psychological burden. In recent years, the focus of
scientists on treating OA has turned to disease
prevention and treatment of early OA. Previous studies
have proved that WuFu Decoction (WFD) could protect
chondrocytes, and the Chinese herbs in this prescription
have shown anti-inflammatory effects. In this study, we
built the OA rat model by the modified Hulth method,
conducted research with WFD, and set D‐Glucosamine
sulfate as the positive control. It is proven that WFD
improved the micromorphology and tissue damage of
the knee joint, decreased the score of the Mankin and
OARSI system, and inhibited the IL-1β, TNF-α, MMP
13, and CTX-1 levels in serum. Additionally, WFD
treatment inhibited p-ERK1/2 levels and raised the
mRNA and protein levels of TGF-β1, p-Smad2, p
Smad3, type II collagen (the gene is COL2A1), ALP,
TRACP, and BMP7. Furthermore, TGF-β1 inhibitor
(Decorin) reversed the improvement effect of WFD on
OA, but ERK1/2 inhibitor (PD98059) promoted this
improvement effect. Silencing TGFβR2 can reverse the
protective effect of WFD on primary chondrocytes. It is
suggested that WFD can improve OA and chondrocytes
by regulating the TGF-β1/Smad and ERK1/2 pathways.
This study also identified active ingredients, such as
Fumaric acid, Glycyrrhizic acid, Albiflorin, and
Isoliquiritigenin. It provides a basis for the clinical
application of WFD and for the promotion and
development of TCM.
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