Publication: Hypomethylation-mediated overexpression of ITGA2 stimulates cell invasion and migration of thyroid carcinoma
Authors
Chen, Hong ; Zhang, Chunying ; Li, Yanbing ; Chen, Chunyou
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Publisher
Universidad de Murcia. Servicio de publicaciones
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DOI
https://doi.org/10.14670/HH-18-552
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info:eu-repo/semantics/article
Description
Abstract
Objective. To study the molecular
mechanism of DNA methylation-mediated ITGA2
overexpression in thyroid carcinoma (TC).
Methods. First, 450K methylation data and mRNA
expression profiles in TCGA-THCA dataset were
downloaded from TCGA database. ITGA2 was
identified as a methylation-driven gene by using R
package “MethylMix”. Afterwards, qRT-PCR, western
blot and flow cytometry assay were performed to
measure ITGA2 expression in TC cells. Methylationspecific PCR was utilized to measure promoter region
methylation of ITGA2 in TC cells. Transwell and wound
healing assays were carried out to assess cell invasive
and migratory properties.
Results. Compared with normal cells, TC cells
presented significantly increased ITGA2 expression. In
addition, ITGA2 expression was controlled by DNA
methylation. Hypomethylation of CpG island resulted in
an increased ITGA2 expression. Hence, methylation and
expression levels of ITGA2 were inversely associated.
Moreover, overexpression of ITGA2 and promoter
region hypomethylation facilitated cell invasive and
migratory abilities in TC.
Conclusion. These findings authenticated that
promoter region hypomethylation of ITGA2 fostered
ITGA2 expression as well as TC cell invasion and
migration.
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Citation
Histology and Histopathology, Vol.38, nº7, (2023)
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