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Aldose reductase B1 in pig seminal plasma: identification, localization in reproductive tissues, and relationship with quality and sperm preservation

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dc.contributor.authorMateo Otero, Yentel
dc.contributor.authorViñolas Vergés, Estel
dc.contributor.authorLlavanera, Marc
dc.contributor.authorRibas Maynou, Jordi
dc.contributor.authorRoca, Jordi
dc.contributor.authorYeste, Marc
dc.contributor.authorBarranco, Isabel
dc.contributor.departmentMedicina y Cirugía Animal
dc.date.accessioned2024-09-06T07:18:47Z
dc.date.available2024-09-06T07:18:47Z
dc.date.issued2021-06-08
dc.descriptionThis manuscript version is made available under the CC-BY 4.0 license http://creativecommons.org/licenses/by/4.0/. This document is the Published version of a Published Work that appeared in final form in Frontiers in Cell and Developmental Biology. To access the final edited and published work see https://doi.org/10.3389/fcell.2021.683199
dc.description.abstractAldose reductase B1 (AKR1B1), a NADPH-dependent enzyme that belongs to the aldo-keto reductase protein superfamily, has been reported to be involved in both male and female reproductive physiology. The objectives of this study were: (1) to evaluate the concentration of SP-AKR1B1 in pig ejaculate fractions; (2) to describe the immunohistochemical localization of AKR1B1 alongside the boar genital tract; (3) to evaluate the relationship between SP-AKR1B1 and sperm quality/functionality parameters. Ejaculates from seven boars (one ejaculate per boar) were collected in separate portions [the first 10 mL of the sperm rich fraction (SRF-P1), the rest of the SRF (SRF-P2), and the post-SRF (PSRF)], and the concentration of SP-AKR1B1 was assessed using an enzyme-linked immunosorbent assay (ELISA). Immunohistochemistry and immunoblotting targeting was conducted in the reproductive tissues of these boars. Additionally, the entire ejaculates of 14 boars (one ejaculate per boar) were collected and split into three separate aliquots for: (i) SP-AKR1B1 quantification; (ii) assessment of sperm concentration and morphology; and (iii) evaluation of sperm quality and functionality parameters upon ejaculate collection (0 h) and after 72 h of liquid storage at 17°C. Concentration of AKR1B1 in the SP of SRF-P1 (458.2 ± 116.33 ng/mL) was lower (P < 0.05) than that of SRF-P2 (1105.0 ± 229.80 ng/mL) and PSRF (1342.4 ± 260.18 ng/mL). Monomeric and dimeric AKR1B1 forms were expressed alongside the reproductive tissues, except in the bulbourethral glands. No relationship between SP-AKR1B1 and sperm quality/functionality parameters was observed either at 0 h or after 72 h of storage at 17°C. In conclusion, AKR1B1 is expressed in the reproductive organs of boars (except bulbourethral glands) and a higher concentration is found in the PSRF suggesting that seminal vesicles would be the main secretory source. However, this enzyme does not appear to be related to sperm quality/functionality or to the sperm ability to withstand liquid storage at 17°C.es
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dc.identifier.citationFront. Cell Dev. Biol. 9:683199
dc.identifier.doihttps://doi.org/10.3389/fcell.2021.683199
dc.identifier.issnElectronic: 2296-634X
dc.identifier.urihttp://hdl.handle.net/10201/143658
dc.languageenges
dc.publisherFrontiers Media
dc.relationThis study was funded by the Ministry of Science and Innovation, Spain (Grants: RYC-2014-15581 and AGL2017-88329-R), European Union’s Horizon 2020 Research and innovation Scheme (H2020-MSCA-IF-2019-891382), the Seneca Foundation Murcia, Spain (19892/GERM-15), and the Regional Government of Catalonia (Grants: 2017-SGR-1229 and 2020-FI-B-00412).es
dc.relation.publisherversionhttps://www.frontiersin.org/journals/cell-and-developmental-biology/articles/10.3389/fcell.2021.683199/fulles
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.rightsAtribución 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectAKR1B1es
dc.subjectAldose reductase B1
dc.subjectEjaculate fractions
dc.subjectPig
dc.titleAldose reductase B1 in pig seminal plasma: identification, localization in reproductive tissues, and relationship with quality and sperm preservationes
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
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