Publication: Differential expression of Yes-associated protein and phosphorylated Yes-associated protein is correlated with expression of Ki-67 and phospho-ERK in colorectal adenocarcinoma
Authors
Kim, Dong-Hoon ; Kim, Seok-Hyung ; Lee, Ok-Jun ; Huang, Song-Mei ; Kwon, Ju-Lee ; Kim, Jin Man ; Kim, Ji-Yeon ; Seong, In Ock ; Song, Kyu Sang ; Kim, Kyung Hee
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Publisher
F. Hernández y Juan F. Madrid. Universidad de Murcia. Departamento de Biología Celular e Histología
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DOI
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info:eu-repo/semantics/article
Description
Abstract
Yes-associated protein (YAP) is a
transcriptional co-activator and functions as a nuclear
downstream effector of the Hippo pathway. Differential
expression of YAP and phosphorylated Yes-associated
protein (pYAP), which are involved in the expression of
Ki-67 and phosphorylated extracellular signal-regulated
kinase (pERK) in colorectal adenocarcinoma (CRAC), is
not clear. Herein, we hypothesized that nuclear
expression of YAP could predict cell proliferation and
poor prognosis, while cytoplasmic expression of pYAP
would show a reverse correlation with cell proliferation.
Paraffin-embedded samples from 144 CRAC patients
were studied using immunohistochemistry for YAP,
pYAP, Ki-67 and pERK. Frozen samples from 20 CRAC
patients were examined for YAP mRNA in tumor and
non-tumor tissues, using quantitative real-time PCR.
High nuclear YAP expression coincided with high Ki-67
expression (P=0.002). The high nuclear YAP expression
group tended to display a poor overall and disease-free
survival (P=0.089 and P=0.089, respectively), but YAP
mRNA levels in the 20 CRAC tissues were not
significantly different in comparison with the 20 nontumor
tissues (P=0.929). We observed an inverse
correlation between high cytoplasmic pYAP expression
and high Ki-67 expression (P=0.001). Nuclear pERK
expression was positively correlated with nuclear YAP
expression, but negatively correlated with cytoplasmic
pYAP expression (P=0.017 and P=0.020, respectively).
Activated nuclear YAP and inactivated cytoplasmic
pYAP in CRAC showed a positive correlation with Ki67
and nuclear pERK expression, suggesting that the
expression of YAP and pYAP is a possible predictor of
tumor cell proliferation and prognosis in CRAC.
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Citation
Histology and Histopathology, vol. 28, nº 11 (2013)
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