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Restorative potential of ciliary body cells in a retinal ganglion cell degeneration model

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dc.contributor.authorFernández-Nogales, Marta
dc.contributor.authorHerrera, Macarena
dc.contributor.authorHerrera, Eloisa
dc.contributor.authorLucas Ruiz, Fernando
dc.contributor.authorValiente Soriano, Francisco Javier
dc.contributor.authorNadal-Nicolás, Francisco Manuel
dc.contributor.authorAgudo Barriuso, Marta
dc.contributor.authorLucas Ruiz, Fernando
dc.contributor.departmentOftalmología, Optometría, Otorrinolaringología y Anatomía Patológica
dc.contributor.otherFacultades de la UMU::Facultad de Medicina
dc.date.accessioned2026-02-03T10:03:11Z
dc.date.available2026-02-03T10:03:11Z
dc.date.copyright© The Author(s) 2025
dc.date.issued2025-05-03
dc.description.abstractThe ciliary body (CB) has been proposed as a niche of neural stem cells because, in vitro, cells from this area are able to form neurospheres, proliferate and differentiate. Here, we explore the potential of CB cells to differentiate and replace degenerated retinal ganglion cells (RGCs) in vivo. CB cells and cells from the subventricular zone (SVZ) were isolated from adult or postnatal C57BL/6Tg(CAG-EGFP) mice, respectively, and intravitreally injected into intact retinas, immediately after optic nerve crush or 45 days after the lesion of adult C57/BL/6 mice. Retinas were analysed in whole mounts or cross sections at different time points. Controls were matched untreated retinas. Neither cell type caused gliosis or toxicity when injected into intact retinas. When CB or SVZ cells were injected right after axotomy, they formed an epimembrane without integrating in the retina. However, when CB cells were administered in retinas depleted of RGCs, they integrated into the ganglion cell layer and expressed RGC and neuronal markers. Although SVZ cells were also able to integrate into RGC depleted retinas they did so more slowly than CB cells. These results shed light in the long-standing question of whether cells in the CB have the potential to transdifferentiate in vivo and point to the CB as a suitable source of cells that could be used in cell-replacement therapies for neurodegenerative diseases of the retina.
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dc.identifier.citationSci Rep. 2025 May 3;15(1):15503.
dc.identifier.doihttps://doi.org/10.1038/s41598-025-00283-0
dc.identifier.urihttp://hdl.handle.net/10201/198669
dc.languageeng
dc.publisherNature Research
dc.relationSpanish Ministry of Economy and Competitiveness, Instituto de Salud Carlos III, Fondo Europeo de Desarrollo Regional “Una Manera de Hacer Europa” PI19/00071, PI24/00040 (MAB). Fundació La Marató de TV3 20142130 (EH) and 20142131 (MAB). The laboratory of E. H. is supported by grants from the Valencia Regional Government (PROMETEO/2020/007), the Spanish Government (PID2022-138245 NB-I00), Fundacion La Caixa (HR21-00824) and Fundacion ICAR (CelMa-ENVEJECE). E. H. laboratory is located at the Instituto de Neurociencias which is a Severo Ochoa Excellence Center CEX2021-001165-S.
dc.relation.publisherversionhttps://www.nature.com/articles/s41598-025-00283-0
dc.rightsAttribution 4.0 International*
dc.rights.accessRightsinfo:eu-repo/semantics/openAccess
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectOptic nerve
dc.subjectRGCs
dc.subjectNeuroprotection
dc.subjectStem cell therapy
dc.subjectCell replacement
dc.subjectAxotomy
dc.subject.odsObjetivo 3: Salud
dc.titleRestorative potential of ciliary body cells in a retinal ganglion cell degeneration model
dc.typeinfo:eu-repo/semantics/article
dc.type.versioninfo:eu-repo/semantics/publishedVersion
dspace.entity.typePublicationes
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