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Pulmonary toxicity of instilled cadmium-doped silica nanoparticles during acute and subacute stages in rats

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dc.contributor.authorCoccini, Teresa
dc.contributor.authorBarni, Sergio
dc.contributor.authorVaccarone, Rita
dc.contributor.authorMustarelli, Piercarlo
dc.contributor.authorManzo, Luigi
dc.contributor.authorRoda, Elisa
dc.date.accessioned2018-02-15T08:41:05Z
dc.date.available2018-02-15T08:41:05Z
dc.date.issued2013
dc.description.abstractPotential risk associated with new nanomaterial exposure needs to be assessed. This in vivo study investigated pulmonary effects of engineered cadmium-containing silica nanoparticles Cd/SiNPs (1 mg/rat), silica SiNPs (600 µg/rat) and CdCl 2 (400 µg/rat) 1, 7 and 30 days after intratracheal instillation. Comprehensive histopathological and immunocytochemical characterization of lung damage in terms of apoptosis, cell proliferation, inflammation, fibrosis and metabolism were obtained. After exposure to all treatments, lung parenchyma showed injury patterns characterized by collapsed alveoli, inflammation, granuloma formation, thickened alveolar septa and bronchiolar epithelium exfoliation. Type II pneumocytes, containing scarcely surfactantlamellated bodies, were also observed. Apoptotic phenomena enhanced as following, Cd/SiNPs>CdCl 2> SiNPs. In parallel with these findings, a significant increase of PCNA-immunoreactive cells was detected together with high mitotic activity. Cellular localization and distribution of IL-6, IP-10 and TGF-ß1 revealed an increased expression of these cytokines as evidence of an enhanced cellular inflammatory response. CYP450- immunoreactivity was also enhanced, at bronchiolar (e.g. Clara cells) and alveolar (e.g. macrophages) level after both Cd/SiNPs and CdCl 2. These overall effects were observed acutely and lasted until the 30th day, with Cd/SiNPs producing the most marked effects. Collagen-immunolabelling changed particularly 7 and 30 days after Cd/SiNPs, when a strong stromal fibrogenic reaction occurred. The present findings suggest that Cd/SiNPs produce significantly greater pulmonary alterations than either SiNPs or CdCl2 under the present experimental conditions.es
dc.formatapplication/pdfes
dc.format.extent15es
dc.identifier.citationHistology and Histopathology, vol. 28, nº 2, (2013)
dc.identifier.issn1699-5848
dc.identifier.issn0213-3911
dc.identifier.urihttp://hdl.handle.net/10201/56123
dc.languageenges
dc.publisherF. Hernández y Juan F. Madrid. Universidad de Murcia: Departamento de Biología Celular e Histologíaes
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.subjectNanotoxicologyes
dc.subjectIn vivoes
dc.subjectApoptosises
dc.subject.otherCDU::6 - Ciencias aplicadas::61 - Medicinaes
dc.titlePulmonary toxicity of instilled cadmium-doped silica nanoparticles during acute and subacute stages in ratses
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
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Histology and histopathology Vol.28, nº 2 (2013)