Publication: Developing the rate equations for two enzymatic Ping-Pong reactions
in series: Application to the bio-synthesis of Bis(2-ethylhexyl) azelate
Authors
Gómez, M. ; Murcia, M.D. ; Serrano-Arnaldos, M. ; Gómez, E. ; Gómez, J.L. ; Hidalgo, A.M. ; Máximo, M.F.
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Publisher
Elsevier
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DOI
https://doi.org/10.1016/j.bej.2020.107691
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info:eu-repo/semantics/article
Description
©2020. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
This document is the Submitted version of a Published Work that appeared in final form in Biochemical Engineering Journal.. To access the final edited and published work see https://doi.org/10.1016/j.bej.2020.107691
Abstract
In this work, the rate equations of two simultaneous bisubstrate Ping-Pong in
series reactions have been developed for the first time. To obtain these equations, the
approximation to the stationary state has been applied and, for the total balance of
enzyme, all the intermediate complexes of the two reactions, which are present
simultaneously in the reaction medium, have been taken into account.
To check the kinetic equations obtained, the synthesis of bis(2-ethylhexyl)
azelate by transesterification from diethyl azelate and 2-ethylhexanol, in the presence of
the immobilized lipase Novozym@ 435, has been used as reaction model. The reaction
has been carried out in solvent-free conditions in a batch reactor. A design model of the
reactor has also been developed and solved by applying a numerical method. The model
equations have been implemented in user’s software in Visual Basic for Applications. In
that software a routine, based on a very well-known procedure to minimize the sum of
the square errors, has also been implemented. By using this software, the model
parameters have been determined by fitting the experimental data to the model. A high
determination coefficient was obtained, which validated both the kinetic equations and
the reactor’s design model.
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