Publication: Immunohistochemical detection of cell proliferation in gastric carcinomas with the monoclonal antibody ki-67. A study of 24 cases
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Date
1993
Authors
Hoang, Catherine ; Polivka, Marc ; Maragi, J.A. ; Valleu, P. ; Nemeth, J. ; Galian, Annie
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
The proliferative activity in 24 gastric
carcinomas was determined by an immunohistochemical
method using monoclonal antibody Ki-67 (ABC
method). Immunostained nuclei were counted by two
observers through a Nachet NS 1000 image numeriser.
Three grades were defined according to stained nuclei
percentage (proliferation index Pi = percentage of cells
engaged in cellular cycle outside Go): grade 1 (Pi <
20%); grade 2 (20% < Pi < 40%); grade 3 (Pi > 40%).
About 60% of tumours were in grade 1 and 10% in
grade 3.
No correlations were observed between Pi and the
following parameters: histological differentiation;
parietal extension; presence or absence of metastasis.
These results may be compared to the two other
avalable studies of Ki-67 antibody in gastric cancers.
Our study also showed a heterogeneous distribution of
immunostained nuclei, within each single tumour and
from one tumour to another, which has been noted in one
previous study and in a similar one we made on
colorectal carcinoma. This heterogeneity is the
consequence of the variability of carcinomatous cell
proliferative activity; an important biological factor in
the evaluation of tumoral process.
The proliferative activity in gastric carcinomas
provides an estimation of tumour dynamics that might be
a prospective criterium for tumoral process evaluation.
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