Publication: Monoclonal antibodies for immunodetection
of fibrin deposits on cancer cells
Authors
Schardt, Friedrich W. ; Schmausser, Bernd ; Bachmann, Eva
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Publisher
F. Hernández y Juan F. Madrid. Universidad de Murcia. Departamento de Biología Celular e Histología
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DOI
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info:eu-repo/semantics/article
Description
Abstract
The progression of a tumor from benign to
malign and localized to invasive and metastatic growth
is the major cause of poor outcome of therapy in cancer
patients. The deposition of fibrin along with other procoagulant
molecules into the extracellular matrix
obviously serves as a scaffold to support proliferation,
migration and tumor cell growth as well as protection
against the immune system.
The use of antibodies as agents for the
immunodetection of fibrin deposits in vivo has been
hampered by anti-fibrin cross-reactivities with
fibrinogen. For the immunohistochemical detection of
fibrin we used highly specific monoclonal antibodies to
a synthetic fibrinunique peptide, because the fibrin
molecule shares many epitopes with fibrinogen. The
monoclonal antibody was applied to adenocarcinoma of
colon, mamma, pancreas, sarcoma and acute myeloic
leukemia. In all tissue sections and cytospin preparations
fibrin was identified in a direct apposition to the surface
membranes of carcinoma and sarcoma cells,
predominantly at the host-tumor interface and also in
regions directly adjacent to zones of angiogenesis,
whereas normal cells and tissue showed no deposits of
fibrin. The findings will be supported by investigations
that factors and components of the coagulation system
could be detected in the tumor stroma and tumor cells.
These factors are obviously produced and secreted by
the malignant cells and deposited together with
fibrinogen into the extracellular matrix. Our results show
that basically all malignant cells examined,
independently of ectodermal or mesenchymal derivation,
themselves are the origin of hypercoagulability and
fibrinolytic system inhibition
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Citation
Histology and Histopathology, vol. 28, nº 8 (2013)
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