Publication: Glial cell line-derived neurotrophic factor expression in the brain of adult zebrafish. Danio rerio
Loading...
Date
2008
Authors
Lucini, C. ; Maruccio, L. ; Patruno, M. ; Tafuri, S. ; Staibano, S. ; Mascarello, F. ; Castaldo, L.
item.page.secondaryauthor
item.page.director
Publisher
Murcia : F. Hernández
publication.page.editor
publication.page.department
DOI
item.page.type
info:eu-repo/semantics/article
Description
Abstract
In mammals, glial cell line-derived
neurotrophic factor (GDNF) is a growth factor of many
neuronal populations in the central, peripheral and
autonomous nervous system. GDNF may also function
as a morphogen during kidney development and may
regulate spermatogonial differentiation. GDNF has been
characterised in zebrafish embryos and was
demonstrated experimentally to be critical for the
development of the enteric nervous system. However, in
adult zebrafish, no data exist regarding GDNF
expression and localisation in the brain and in different
organs. Thus, the aim of the present study was to
investigate the expression of GDNF in the brain of adult
zebrafish (Danio rerio).
Transcripts of GDNF mRNA were observed in brain
extracts by a standard RT-PCR. The presence of the
protein in the brain homogenates was confirmed by
SDS-PAGE electrophoresis and Western blotting
analysis. Immunohistochemistry and in situ
hybridization experiments showed that GDNF protein
and mRNA were localised in various nuclei of the
telencephalon, diencephalon, mesencephalon,
cerebellum and medulla oblongata of the zebrafish brain.
In conclusion, this study showed that the expression of GDNF was not restricted to developmental periods but it
seems that this factor might be involved in adult
zebrafish brain physiology, as observed in mammals.
publication.page.subject
Citation
item.page.embargo
Ir a Estadísticas
Sin licencia Creative Commons.