Publication: Exoglycosidases and lectins as sequencing approaches of salivary gland oligosaccharides
Authors
Menghi, Giovanna ; Materazzi, G.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
This review was focused on the salivary
gland oligosaccharide chains studied by lectin
histochemistry combined with exoglycosidase digestion.
Glycoconjugates play an important role in many
biofunctions and, generally. salivary mucins, which
consist of numerous oligosaccharide chains attached at
closely spaced intervals to a peptide backbone, serve as
lubricants and protective agents, but in many instances
we are ignorant about the role of biochemically
identified oligosaccharides. Lectin histochemistry
represents the greatest analytic tool to study
carbohydrates in situ; in addition, there is availability of
selective enzymes, so glycosidase degradation is useful
to both investigate the structure of a given oligosaccharide
and verify the influence of neighbouring
sugars on the affinity towards the respective specific
lectins. Using stepwise digestion of samples, followed
by lectin labelling, the structure of terminal short
oligosaccharides with blood-group activity was also
elucidated. Additional histochemical methodologies
were developed to establish the presence of acetylated
groups in sialic acid residues, and the position of the
linkage to the underlying monosaccharide. Sequencing
approaches by exoglycosidases and lectins were also
seen to be particularly useful when substantial
differences did not emerge in lectin affinity,
glycoconjugate composition and complex carbohydrate
cytochemistry.
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