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Hyperlipidemic chicken as a model of non-alcoholic steatohepatitis

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dc.contributor.authorAyala, Ignacio
dc.contributor.authorMartin Castillo, Antonia
dc.contributor.authorAdánez Martínez, María de Gracia
dc.contributor.authorFernández-Rufete Navarro, Ana
dc.contributor.authorGarcía Pérez, Bartolomé
dc.contributor.authorCastells Mora, María Teresa
dc.contributor.departmentMedicina
dc.date.accessioned2025-01-21T12:16:24Z
dc.date.available2025-01-21T12:16:24Z
dc.date.issued2009-01-01
dc.description© 2009 by the Society for Experimental Biology and Medicine. This manuscript version is made available under the CC-BY 4.0 license http://creativecommons.org/licenses/by/4.0/. This document is the Accepted version of a Published Work that appeared in final form in Experimental Biology and Medicine. To access the final edited and published work see https://doi.org/10.3181/0807-RM-219
dc.description.abstractNon-alcoholic steatohepatitis (NASH) is part of the spectrum of non-alcoholic fatty liver disease (NAFLD), currently the most common cause of abnormal liver tests. Given the difficulty of studying all the factors involved in it in human populations, studies in animal models might provide crucial insights in the pathogenesis of steatohepatitis. Several physiological features predispose birds to fat deposition in the liver. The present study was conceived to explore the possibilities of the chicken fed a cholesterol and fat enriched diet as a model for steatohepatitis. We used two different diets: a standard growing mash (control group) and a standard growing mash enriched with 2% cholesterol and 20% palm oil (hyperlipidemic group). We investigated the effect of feeding a cholesterol and fat enriched diet, on plasma lipid levels, liver enzymes and hepatic histopathology. Semiquantitative and quantitative assessment by image analysis was performed to determine changes in lipid deposits and inflammatory infiltration. Statistically significant increases were observed in all plasma lipid parameters, liver macroscopic features, fat deposits and cell-ballooning of hepatocytes between control and hyperlipidemic animals. Significant differences were also observed in the inflammatory infiltration parameters (number of foci, density, area and maximal diameter). Results show that diet-induced hypercholesterolemia and hypertriglyceridemia are associated with severe impairment of liver histology (fat accumulation, inflammation and cell-ballooning), reproducing histological features of human NAFLD. This model, which is easy and reproducible, offers economic and technical advantages. Furthermore, the reversibility of the pathologic changes makes it suitable for drug intervention studies of steatohepatitis.es
dc.formatapplication/pdfes
dc.format.extent8
dc.identifier.citationExperimental Biology and Medicine. 2009, 234(1):10-16
dc.identifier.doihttps://doi.org/10.3181/0807-RM-219
dc.identifier.issnPrint: 1535-3702
dc.identifier.issnElectronic: 1535-3699
dc.identifier.urihttp://hdl.handle.net/10201/148911
dc.languageenges
dc.publisherFrontiers Media
dc.relationThis research was funded by Grants 05671/PI/07 and 04542/GERM/06 from Fundación Seneca (Programa de Generación de Conocimiento Científico de Excelencia y Ayudas a Grupos de Excelencia de la Región de Murcia, de la Fundación Seneca, Agencia de Ciencia y Tecnologí de la Región de Murcia, Plan Regional de Ciencia y Tecnología 2007/2010, Spain).es
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.rightsAtribución 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectNon-alcoholic steatohepatitis (NASH)
dc.subjectChicken
dc.subjectExperimental animal model
dc.titleHyperlipidemic chicken as a model of non-alcoholic steatohepatitises
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
relation.isAuthorOfPublicationd6ad2235-d5ca-4574-85eb-964da3c5c44c
relation.isAuthorOfPublication.latestForDiscoveryd6ad2235-d5ca-4574-85eb-964da3c5c44c
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