Publication: Hippocampal expressions of metallothionein I/II and glycoprotein 96 in EAE-prone and EAE-resistant strains of rats
Authors
Grubić Kezele, Tanja ; Blagojević Zagorac, Gordana ; Jakovac, Hrvoje ; Domitrović, Robert ; Radošević Stašić, Biserka
item.page.secondaryauthor
item.page.director
Publisher
Universidad de Murcia. Departamento de Biología Celular e Histología
publication.page.editor
publication.page.department
DOI
DOI: 10.14670/HH-11-780
item.page.type
info:eu-repo/semantics/article
Description
Abstract
Inflammatory demyelinating diseases such as
multiple sclerosis and experimental autoimmune
encephalomyelitis (EAE) are often followed by
cognitive deficits associated with the neuronal injury,
synaptic loss and altered neurogenesis within the
hippocampus. Changes depend on the genetic and
epigenetic factors that ensure the cellular and
environmental homeostasis and regulate the interactions
of immunocompetent, glial and neural cells. Owing to
high impact of stress proteins on these processes, in this
study we compared the protein content of interleukin-6,
transforming growth factor-β1, metallothioneins I/II
(MTs) and glycoprotein 96 (gp96) in the hippocampus of
DA and AO rats that differ in the susceptibility to the
induction of EAE, and tested the relationship of MTs and
gp96 to granule neurons, glial cells and neural
progenitors in different subfields of dentate gyrus. Rats
were immunized with bovine brain homogenate
emulsified in complete Freund’s adjuvant or only with
CFA. The data showed that acute attack of EAE in DA
rats was followed by accumulation of IL-6, TGF-β1 and
MTs proteins, by increased expression of MTs in
molecular and granular cell layer, by reduced expression
of gp96/granular cell, by apoptosis and by microgliosis
with appearance of Iba-1+ cells, co-expressing MT I/II
and gp96. Furthermore, in subgranular zone (SGZ) of
DA rats an augmented number of GFAP+ precursors, but
decreased number of doublecortin (DCX)+ neuroblasts
and immature NeuN+ neurons were found, implying that
in DA rats the neurogenesis was delayed or reduced.
Besides, in SGZ of both strains several DCX+ and
NeuN+ cells co-expressing gp96 and MT I/II were
found.
publication.page.subject
Citation
Histology and Histopathology, Vol.32, nº2, (2017)
item.page.embargo
Ir a Estadísticas
Este ítem está sujeto a una licencia Creative Commons. http://creativecommons.org/licenses/by-nc-nd/4.0/