Publication: Field emission SEM, conventional TEM and HVTEM study of submandibular gland in prenatal and postnatal aging mouse
Loading...
Date
1997
Authors
Watanabe, I. ; Jin, C. ; Nagata, T.
item.page.secondaryauthor
item.page.director
Publisher
Murcia : F. Hernández
publication.page.editor
publication.page.department
DOI
item.page.type
info:eu-repo/semantics/article
Description
Abstract
The development of acinar and ductal cells
of the mouse submandibular gland was studied using
field emission SEM, conventional TEM and HVTEM
methods. The specimens, at 15 and 18 days of gestation
and 1, 3, 7, 14, 21, 30, 90 and 180 postnatal days were
fixed in 2.5% glutaraldehyde solution in 0.1M sodium
phosphate buffer (pH 7.3). At 15 and 18 days of
gestation, the structure of mouse submandibular gland
contains acinar and ductal cells in proliferation. The
cytoplasmic organelles such as mitochondria, granular
endoplasmic reticulum and Golgi apparatuses are
scattered in the cytoplasm. At 18 prenatal days only
severa1 acinar cells present immature secretory granules
in the apical portion. In this stage the acinar and ductal
cells are enveloped by bundles of fine collagen fibrils
disposed in severa1 directions. There are also numerous
capillaries located closely to the acinar cell membranes.
In the aging stages of 1, 3, 7, 14, 21, and 30 postnatal
days, the histo-differentiation of acinar, intercalated and
ductal cell components are observed. At newborn day
one the cytoplasmic organelles start to place themselves
around the nucleus. Severa1 immature secretory granules
are observed at day one, however, they increase in the
aging days. At postnatal day 30, the cytoplasms of acinar
and ductal cells are filled with a large number of
secretory granules of different sizes. The stacks of
granular endoplasmic reticulum and Golgi apparatus and
some vesicles and free ribossomes are noted. The
intercellular membranes are attached by desmosomes
and cytoplasmic interdigitations. The luminal surface
shows severa1 small projections of microvilli. An
electron-dense line of basement membranes followed by
fine collagen fibrils are recognized. Delicate capillaries
are found in the outer surface of acinar cells. At
postnatal day 90 and 180 the acinar, intercalated and
striated ductal cells reveal numerous secretory granules
Offprint requests to: Prof. Dr. l. Watanabe, Department of Anatomy,
lnstitute of Biomedical Sciences, University of Sao Paulo, Cep 05508-
900 Cidade Universitária, Sao Paulo, Brazil
in the apical portion. The acinar cells showed basa1
nuclei and the parallel arrangement of granular
endoplasmic reticulum. The mitochondria are located at
the base of ductal cells showing a typical pattern of
cristae. In these stages the intercellular digitations of
cytoplasmic protrusions and desmosomes are also noted.
The cytoplasm of myoepithelial cells are seen along the
cell membranes. The spongy-like stmctures constituting
the basement membrane are followed by bundles of fine
collagen fibers.
publication.page.subject
Citation
item.page.embargo
Ir a Estadísticas
Sin licencia Creative Commons.