Publication: Quantification of the heterogeneity of cytokeratin 18 immunoexpression in prostate adenocarcinoma and normal prostate: Global and local features
Authors
Santamaría, Luis ; Ingelmo, Ildefonso ; Sinues, Bryan ; Martínez, Laura ; Teba, Fernando
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Publisher
Universidad de Murcia. Departamento de Biología Celular e Histología
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DOI
DOI: 10.14670/HH-18-009
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info:eu-repo/semantics/article
Description
Abstract
There are few studies comparing global
versus local changes in spatial patterns in prostate
cancer. In this study, stereological tools have been
applied to find out if the cytokeratin18 (ck18)
immunoexpression shows local changes in cancer
compared to normal prostate. To verify if these changes
are relevant to ascertain differences between normal
(CTR) and cancer (Ca) cases, several parameters were
estimated. Volume fraction of epithelium immunostained
for ck18 (VV ck18), dispersion index of VV ck18,
positional variance of VV ck18, and multiscale entropy
analysis (MSE) to measure the tissue heterogeneity. The
MSE values showing significant differences between
CTR and Ca were employed in a discriminant analysis to
determine if MSE was able to classify the cases in CTR
and Ca groups. The findings obtained indicate that
changes in the expression of ck18 by the cancer prostate
are heterogeneous. The increase in local variability of
ck18 immunoexpression can be related to the increase in
heterogeneity of shape and size of the tumor acini. The
asymmetry of distribution of the local values of VV ck18
along the axis of the space series may indicate the
existence of anisotropy in the distribution of tumor acini.
The increase in scale-dependent entropy for VV ck18 in
cancer at the morphological level could be interpreted as
the macroscopic expression of the same increase at the
molecular level already described. The discriminant
analysis shows that the dependence on the resolution for
MSE values need to be taken into account to
characterize the prostate cancer better
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Citation
Histology and Histopathology, Vol.33, nº10, (2018)
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