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Fluorescent Bioinspired Protein Labeling with Betalamic Acid. Derivatization and Characterization of Novel Protein-Betaxanthins

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dc.contributor.authorFrancisco, García-Carmona
dc.contributor.authorMercedes, Jiménez-Atiénzar
dc.contributor.authorCabanes Cos, Juana
dc.contributor.authorEscribano Cebrián, Josefa
dc.contributor.authorGandía Herrero, Fernando
dc.contributor.departmentBioquímica y Biología Molecular Aes
dc.date.accessioned2021-02-11T12:58:18Z
dc.date.available2021-02-11T12:58:18Z
dc.date.issued2016-10
dc.description.abstractBetaxanthins are the water-soluble pigments that bestow yellow coloration to fruits, flowers and roots of plants of the Caryophyllales order and present autofluorescence after excitation with blue light. In this work, the semi-synthesis of betaxanthins derived from macromolecules is achieved for the first time by exploiting the reactivity of amine groups belonging to proteins. The synthesis of protein-betaxanthins is demonstrated by spectrophotometry and HPLC-ESI-TOF-MS mass analysis. The derivatization with betalamic acid was in a ratio 1:1 and yielded protein-betaxanthins yellow in color that exhibited fluorescent properties with a maximum excitation wavelength of 476 nm and a maximum emission wavelength of 551 nm. Moreover, staining can be started from purified betalamic acid or directly from raw red beet root extracts. The novel bioinspired labeling reaction allowed protein detection in conventional fluorescence scanning and imaging systems and opens a new perspective for betalamic acid derived molecules as fluorescent probes with multiple biological applications.es
dc.formatapplication/pdfes
dc.format.extent36es
dc.identifier.citationDyes and Pigments, 2016, 133, 458-466
dc.identifier.doihttps://doi.org/10.1016/j.dyepig.2016.06.037
dc.identifier.issn0143-7208
dc.identifier.urihttp://hdl.handle.net/10201/103101
dc.languageenges
dc.relationMinisterio de Economia y Competitividad” (MEC, FEDER, Spain) (Project AGL2014-57431) and by “Programa de ayudas a Grupos de Excelencia de la Región de Murcia, de la Fundación Séneca, Agencia de Ciencia y Tecnología de la Región de Murcia”.es
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.rightsAtribución-NoComercial-SinDerivadas 3.0 España*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/es/*
dc.subjectBetalainses
dc.subjectBetalamic acides
dc.subjectFluorescencees
dc.subjectLabelinges
dc.subjectPigmentses
dc.subjectBioinspiredes
dc.subject.otherCDU::5 - Ciencias puras y naturaleses
dc.titleFluorescent Bioinspired Protein Labeling with Betalamic Acid. Derivatization and Characterization of Novel Protein-Betaxanthinses
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
relation.isAuthorOfPublication14e0a51b-b9d3-49d0-bc89-ca3ba64290dc
relation.isAuthorOfPublicationb34d208f-4344-4241-a476-fcc70f814dc1
relation.isAuthorOfPublicationdb7a1a52-fec7-48eb-91e0-abe5e715b483
relation.isAuthorOfPublication.latestForDiscovery14e0a51b-b9d3-49d0-bc89-ca3ba64290dc
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