Publication: lmmunocytochemistry of perinatal rat livers with a special reference to the roles of mesenchymal cells in hepatic differentiation
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Date
1996
Authors
Hayashida, T. ; Nagata, Kengo ; Doi, Y. ; Ozaka, T. ; Itoh, Hiroyuki
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
To investigate the roles of extracellular
matrix produced by hepatic mesenchymal cells in the
organization of hepatic cell cords, perinatal rat livers
were examined with immunocytochemistry of
fibronectin (FN) and larninin (LM). Some hepatocytes in
a free state at prenatal day 15 actively produced FN and
LM in the rough endoplasmic reticulum but lost this
synthetic activity when such cells were incorporated into
hepatic cell cords. On the other hand, hepatic
mesenchymal cells, especially those associated with the
perisinusoidal space, retained this synthetic activity
throughout the stages examined. In the differentiating
hepatic cell cords, positive imrnunoreactions for FN and
LM were preferentially seen on the cell surface facing
both sinusoidal space and differentiating bile canaiiculus
concomitant with the expression of the tight junction
protein, ZO-1, from prenatal day 17. Since such
hepatocytes have lost or reduced their synthetic activities
of both glycoproteins in the rER, the immunoreactions
appear to be mainly due to hepatic mesenchymal cells
which seem to play a role in the formation of the hepatic
cell cords and the bile canaliculi.
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