Publication: LncRNA CERS6-AS1, sponging miR-6838-5p, promotes proliferation and invasion in cervical carcinoma cells by upregulating FOXP2
Authors
Yan, Kun ; Hu, Chunyan ; Liu, Chen ; Chu, Guanghua ; Wang, Xinru ; Ma, Shuyun ; Li, Long
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Publisher
Universidad de Murcia. Departamento de BiologĂa Celular e HistologĂa
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DOI
https://doi.org/10.14670/HH-18-555
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info:eu-repo/semantics/article
Description
Abstract
Cervical cancer (CC) is a common disease in
women characterized by high recurrence rate. LncRNA
ceramide synthase 6 antisense RNA 1 (CERS6-AS1) has
been found to play a crucial role in the progression of
breast cancer and pancreatic cancer. Nevertheless, the
regulatory role of CERS6-AS1 in CC remains largely
unclear. Here, we found that the expression of CERS6-
AS1 was upregulated in CC tissues and cell lines
compared with adjacent tissues and normal human
cervical epithelial cells. CERS6-AS1 overexpression
promoted proliferation and invasion, and inhibited
apoptosis in CC cells, while silencing of CERS6-AS1
led to the opposite results. CERS6-AS1 was verified as a
sponge of miR-6838-5p by RNA pull-down and
luciferase reporter gene assays. Functional investigations
revealed that CERS6-AS1 knockdown inhibited
proliferation and invasion, and promoted apoptosis in
CC cells, which was reversed by miR-6838-5p inhibitor.
Furthermore, forkhead box P2 (FOXP2) was identified
as a target for miR-6838-5p, and overexpression of miR6838-5p decreased the expression level of FOXP2.
Besides, CERS6-AS1 was able to sponge miR-6838-5p
to accelerate CC cell proliferation and invasion and
inhibited cell apoptosis through upregulating FOXP2
expression. In general, CERS6-AS1 was able to regulate
CC cell proliferation, invasion and apoptosis by the
miR-6838-5p/FOXP2 axis, which suggested that
CERS6-AS1 may be a potential target for the treatment
of CC.
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Citation
Histology and Histopathology, Vol.38, nÂş7, (2023)
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Este Ătem está sujeto a una licencia Creative Commons. http://creativecommons.org/licenses/by-nc-nd/4.0/