Publication: Daily rhythms of lipid metabolic gene expression in zebrafish liver : Response to light/dark and feeding cycles
Authors
Paredes, Juan Fernando ; López Olmeda, José Fernando ; Martínez López, Francisco Javier ; Sánchez Vázquez, Francisco Javier
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Publisher
Taylor and Francis Group [Commercial Publisher]
Taylor and Francis [Imprint]
Taylor and Francis [Imprint]
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DOI
https://doi.org/10.3109/07420528.2015.1104327
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info:eu-repo/semantics/article
Description
©<2015>. This manuscript version is made available under the CC-BY license http://creativecommons.org/licenses/ccby/4.0/
This document is the acepted version of a Published Work that appeared in final form in [Chronobiology International]. To access the final edited and published work see [https://doi.org/ 10.3109/07420528.2015.1104327]
Abstract
Despite numerous studies about fish nutrition and lipid metabolism, very little is known about the daily rhythm
expression of lipogenesis and lipolysis genes. This research aimed to investigate the existence of daily rhythm
expressions of the genes involved in lipid metabolism and their synchronization to different light/dark (LD) and
feeding cycles in zebra fish liver. For this purpose, three groups of zebra fish were submitted to a 12:12 h LD cycle. A
single daily meal was provided to each group at various times: in the middle of the light phase (ML); in the middle of
the dark phase (MD); at random times. After 20 days of acclimation to these experimental conditions, liver samples
were collected every 4 h in one 24-h cycle. The results revealed that most genes displayed a significant daily rhythm
with an acrophase of expression in the dark phase. The acrophase of lipolytic genes (lipoprotein lipase – lpl,
peroxisome proliferator-activated receptor – ppar and hydroxyacil CoA dehydrogenase – hadh) was displayed
between ZT 02:17 h and ZT 18:31 h. That of lipogenic genes (leptin-a – lepa, peroxisome proliferator-activated
receptor – ppar , liver X receptor – lxr, insulin-like growth factor – igf1, sterol regulatory element-binding protein –
srebp and fatty acid synthase – fas) was displayed between ZT 15:25 h and 20:06 h (dark phase). Feeding time barely
influenced daily expression rhythms, except for lxr in the MD group, whose acrophase shifted by about 14 h
compared with the ML group (ZT 04:31 h versus ZT 18:29 h, respectively). These results evidence a strong
synchronization to the LD cycle, but not to feeding time, and most genes showed a nocturnal acrophase. These
findings highlight the importance of considering light and feeding time to optimize lipid metabolism and feeding
protocols in fish farming.
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Citation
Chronobiology International, 32:10, 1438-1448 (2015)
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Este ítem está sujeto a una licencia Creative Commons. http://creativecommons.org/licenses/by/4.0/