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The origin of lipofuscin in brown adipocytes of hyperinsulinaemic rats: the role of lipid peroxidation and iron

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dc.contributor.authorMarkelic, Milica
dc.contributor.authorVelickovic, Ksenija
dc.contributor.authorGolic, Igor
dc.contributor.authorKlepal, Waltraud
dc.contributor.authorOtasevic, Vesna
dc.contributor.authorStancic, Ana
dc.contributor.authorJankovic, Aleksandra
dc.contributor.authorVucetic, Milica
dc.contributor.authorBuzadzic, Biljana
dc.contributor.authorKorac, Bato
dc.contributor.authorKorac, Aleksandra
dc.date.accessioned2018-03-09T18:25:16Z
dc.date.available2018-03-09T18:25:16Z
dc.date.issued2013
dc.description.abstractThe aim of this study was to investigate lipofuscin origin in brown adipocytes of hyperinsulinaemic rats and the possible role of lipid peroxidation and iron in this process. Ultrastructural examination revealed hyperinsulinaemia-induced enhancement in the lipofuscin production, accompanied by an increase of mitochondrial damage in brown adipocytes. Extensive fusions of lipid droplets and mitochondria with lysosomes were also observed. Confocal microscopy showed lipofuscin autofluorescence emission in brown adipose tissue (BAT) after excitation at 488 nm and 633 nm, particularly in the insulin-treated groups. The presence and distribution of lipid peroxidation product, 4-hydroxy-2-nonenal (4- HNE), in brown adipocytes was assessed by immunohistochemical examination revealing its higher content after treatment with insulin. The iron content was quantified by electron dispersive X-ray analysis (EDX) showing its higher content in the hyperinsulinaemic groups. The ultrastucture of the majority of lipofuscin granules suggests their mitochondrial origin, which was additionally confirmed by their colocalization with ATP synthase. In conclusion, our results suggest that increased lipofuscinogenesis in the brown adipocytes of hyperinsulinaemic rats is a consequence of lipid peroxidation, mitochondrial damage and iron accumulation.es
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dc.format.extent11es
dc.identifier.citationHistology and histopathology, Vol. 28, n.º 4 (2013)
dc.identifier.issn1699-5848
dc.identifier.issn0213-3911
dc.identifier.urihttp://hdl.handle.net/10201/57131
dc.languageenges
dc.publisherF. Hernández y Juan F. Madrid. Universidad de Murcia. Departamento de Biología Celular e Histologíaes
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.subjectBrown adipocytees
dc.subjectLipofuscines
dc.subject.otherCDU::5 - Ciencias puras y naturales::57 - Biología::576 - Biología celular y subcelular. Citologíaes
dc.titleThe origin of lipofuscin in brown adipocytes of hyperinsulinaemic rats: the role of lipid peroxidation and irones
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
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Histology and histopathology Vol.28, nº 4 (2013)