Publication: Topological differences along mammalian motor nerve terminals for spontaneous and alpha-Bungarotoxin-induced sprouting
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Date
2000
Authors
Tomas, J. ; Lanuza, M.A. ; Santafe, M. ; Fenoll-Brunet, M.R. ; García, N .
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
Spontaneous sproutings can be observed in
end plates from normal adult vertebrate muscles and
motor end plates develop increased growth signs and
sprouts when target muscle cells become less active or
paralysed. Nevertheless, very little is known about
where in the motor nerve terminal arborization
spontaneous and experimentally induced sprouts
originate, their similarities and differences and also
about their final maturation or elimination. In this study
we investigate the topological properties of both
spontaneous and alpha-bungarotoxin-induced sprouts
(during different periods of intoxication and after
recovery) along the motor nerve terminal branches of the
Levator auris longus muscle of Swiss mice (between 48-
169 day old).
Muscles were processed for immunocytochemistry
to simultaneously detect postsynaptic AChRs and axons.
This procedurk permits us to make an accurate
identification of the fine sprouts and a morphometric
study of the presynaptic branching pattern profile in
control muscles, during the toxin action and after
recovery from paralysis.
The results show that in normal muscles, the initial
and trunk segments (those between branch points) of the
terminal arborization sprouted proportionally more
branches when taking their relative lengths into account
than the distal free-end segments. In contrast, every micrometer of alpha-bungarotoxin-treated muscles
throughout the full terminal arborization have the same
probability of generating a sprout. Moreover, the toxininduced
sprouts can consolidate as new branches once
recovered from the paralysis without changing the total
length of the nerve terminal arborization.
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