Publication: Traditional Chinese medicine Yiqi Huoxue recipe attenuates hepatic fibrosis via YAP/TAZ signaling
Authors
Zhao, Wen ; Zhang, Xiaoxiao ; Hou, Mengmeng ; Zhang, Yuguo ; Tang, Yuhui ; Li, Lu ; Dong, Shiming ; Liu, Lingdi ; Zhao, Dandan ; Li, Wencong ; Nan, Yuemin
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Publisher
Universidad de Murcia, Departamento de Biologia Celular e Histiologia
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DOI
https://doi.org/10.14670/HH-18-373
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info:eu-repo/semantics/article
Description
Abstract
Background/Aims. The Yiqi Huoxue
(YQHX) recipe has been shown to attenuate liver
fibrosis, but precise mechanisms have not yet been
elucidated. Recently, Yes-associated protein
(YAP)/transcriptional coactivator with PDZ-binding
motif (TAZ) signaling has been implicated in liver
fibrogenesis. This study investigated whether the
YAP/TAZ signaling is involved in the therapeutic effect
of YQHX on hepatic fibrosis.
Materials and Methods. Wistar rats were used to
generate a model of carbon tetrachloride (CCl
4)-induced
liver fibrosis. Chronic hepatitis B (CHB) patients with
liver fibrosis were enrolled and assigned to receive either
nucleoside/nucleotide analogues (NAs) or NAs plus
YQHX. Histology, immunohistochemistry, qRT-PCR,
and western blotting were conducted to mechanistically
assess the therapeutic effects of YQHX on liver fibrosis.
Results. YQHX markedly alleviated morphological
alterations in CCl
4-induced liver fibrosis and decreased
markers of hepatic fibrosis in rats. Furthermore, YQHX
significantly suppressed CCl
4-meidated activation of the
transforming growth factor-beta (TGF-β)/Smad
signaling pathway. Notably, CCl
4 induced up-regulation
of YAP, TAZ, and connective tissue growth factor
(CTGF), which were significantly abrogated by YQHX.
Consistent with the above major findings in rats, CHB
patients treated with NAs plus YQHX had greater
improvement in liver fibrosis than those given NAs
alone (71.4% vs. 28.6%; P=0.057). In addition, hepatic
and plasma levels of YAP were significantly decreased
after YQHX treatment in CHB patients with liver
fibrosis.
Conclusion. YAP/TAZ signaling plays a role, at least
in part, in the anti-fibrotic activity of YQHX. The
findings may help to better understand the mechanisms
of YQHX in the treatment of liver fibrosis
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Citation
Histology and Histopathology Vol. 36, nº9 (2021)
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